Oncotarget

Research Papers:

Cyclin D1 represses peroxisome proliferator-activated receptor alpha and inhibits fatty acid oxidation

Sushama Kamarajugadda, Jennifer R. Becker, Eric A. Hanse, Douglas G. Mashek, Mara T. Mashek, Anna M. Hendrickson, Lisa K. Mullany and Jeffrey H. Albrecht _

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Oncotarget. 2016; 7:47674-47686. https://doi.org/10.18632/oncotarget.10274

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Abstract

Sushama Kamarajugadda1,*, Jennifer R. Becker2,*, Eric A. Hanse2, Douglas G. Mashek3, Mara T. Mashek3, Anna M. Hendrickson2, Lisa K. Mullany2, Jeffrey H. Albrecht1

1Gastroenterology Division, Minneapolis VA Health Care System, Minneapolis, MN 55417, USA

2Minneapolis Medical Research Foundation, Minneapolis, MN, 55404, USA

3Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA

*These authors contributed equally to this work

Correspondence to:

Jeffrey H. Albrecht, email: [email protected]

Keywords: breast cancer, hepatocellular carcinoma, liver regeneration, metabolism, peroxisome proliferator-activated receptor alpha

Received: February 08, 2016     Accepted: June 04, 2016     Published: June 24, 2016

ABSTRACT

Cyclin D1 is a cell cycle protein that promotes proliferation by mediating progression through key checkpoints in G1 phase. It is also a proto-oncogene that is commonly overexpressed in human cancers. In addition to its canonical role in controlling cell cycle progression, cyclin D1 affects other aspects of cell physiology, in part through transcriptional regulation. In this study, we find that cyclin D1 inhibits the activity of a key metabolic transcription factor, peroxisome proliferator-activated receptor α (PPARα), a member of nuclear receptor family that induces fatty acid oxidation and may play an anti-neoplastic role. In primary hepatocytes, cyclin D1 inhibits PPARα transcriptional activity and target gene expression in a cdk4-independent manner. In liver and breast cancer cells, knockdown of cyclin D1 leads to increased PPARα transcriptional activity, expression of PPARα target genes, and fatty acid oxidation. Similarly, cyclin D1 depletion enhances binding of PPARα to target sequences by chromatin immunoprecipitation. In proliferating hepatocytes and regenerating liver in vivo, induction of endogenous cyclin D1 is associated with diminished PPARα activity. Cyclin D1 expression is both necessary and sufficient for growth factor-mediated repression of fatty acid oxidation in proliferating hepatocytes. These studies indicate that in addition to playing a pivotal role in cell cycle progression, cyclin D1 represses PPARα activity and inhibits fatty acid oxidation. Our findings establish a new link between cyclin D1 and metabolism in both tumor cells and physiologic hepatocyte proliferation.


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