LINC00052 regulates the expression of NTRK3 by miR-128 and miR-485-3p to strengthen HCC cells invasion and migration
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Dongmei Xiong1,2,*, Yanrui Sheng3,*, Shijia Ding4,*, Juan Chen1, Xixi Tan1, Tao Zeng1, Dongdong Qin3, Liying Zhu1, Ailong Huang1,2 Hua Tang1
1Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China
2Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou, China
3Department of Clinical Laboratory, Jining No.1 People’s Hospital, Jining, China
4Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, China
*These authors contributed equally to this work
Hua Tang, email: [email protected]
Ailong Huang, email: [email protected]
Keywords: HCC, LINC00052, NTRK3, invasion, migration
Received: September 20, 2015 Accepted: June 06, 2016 Published: June 23, 2016
Long non-coding RNAs (LncRNAs) are a group of RNAs that are more than 200 nt in length but cannot encode proteins. Accumulating evidences showed that abnormal LncRNA expressions are highly involved in many kinds of tumor. By using gene trap methods which could knockdown gene expression to find important genes, we found one LncRNA which called intergenic non-protein coding RNA 52 (LINC00052) has the ability to inhibit invasion and migration of hepatocarcinoma cells. We found that invasion, migration and proliferation abilities in SMMC7721 cell were inhibited after up-expressing LINC00052. We identified that NTRK3 was the target gene of LINC00052. Down-expression of NTRK3 could increase SMMC7721 cell invasion, migration and proliferation. Meanwhile, we discovered that LINC00052 could regulate NTRK3 expression by forming complementary base pairing with miR-128 and miR-485-3p to reduce the luciferase activity of NTRK3 3′UTR. These results reveal a new mechanism for understanding hepatocarcinoma cells invasion and migration.
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