Oncotarget

Research Papers:

Comprehensive analysis of lncRNAs microarray profile and mRNA–lncRNA co-expression in oncogenic HPV-positive cervical cancer cell lines

LingYun Yang, Ke Yi, HongJing Wang, YiQi Zhao and MingRong Xi _

PDF  |  HTML  |  How to cite  |  Order a Reprint

Oncotarget. 2016; 7:49917-49929. https://doi.org/10.18632/oncotarget.10232

Metrics: PDF 1644 views  |   HTML 2082 views  |   ?  


Abstract

LingYun Yang1, Ke Yi1, HongJing Wang1, YiQi Zhao1, MingRong Xi1

1Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China

Correspondence to:

MingRong Xi, email: xmrjzz@126.com

Keywords: lncRNA, expression profile, coding non-coding gene co-expression, oncogenic HPV, cervical cancer

Received: February 07, 2016     Accepted: May 16, 2016     Published: June 23, 2016

ABSTRACT

Long non-coding RNAs are emerging to be novel regulators in gene expression. In current study, lncRNAs microarray and lncRNA-mRNA co-expression analysis were performed to explore the alternation and function of lncRNAs in cervical cancer cells. We identified that 4750 lncRNAs (15.52%) were differentially expressed in SiHa (HPV-16 positive) (2127 up-regulated and 2623 down-regulated) compared with C-33A (HPV negative), while 5026 lncRNAs (16.43%) were differentially expressed in HeLa (HPV-18 positive) (2218 up-regulated and 2808 down-regulated) respectively. There were 5008 mRNAs differentially expressed in SiHa and 4993 in HeLa, which were all cataloged by GO terms and KEGG pathway. With the help of mRNA-lncRNA co-expression network, we found that ENST00000503812 was significantly negative correlated with RAD51B and IL-28A expression in SiHa, while ENST00000420168, ENST00000564977 and TCONS_00010232 had significant correlation with FOXQ1 and CASP3 expression in HeLa. Up-regulation of ENST00000503812 may inhibit RAD51B and IL-28A expression and result in deficiency of DNA repair pathway and immune responses in HPV-16 positive cervical cancer cell. Up-regulation of ENST00000420168, ENST00000564977 and down-regulation of TCONS_00010232 might stimulate FOXQ1 expression and suppress CASP3 expression in HPV-18 positive cervical cancer cell, which lead to HPV-induced proliferation and deficiency in apoptosis. These results indicate that changes of lncRNAs and related mRNAs might impact on several cellular pathways and involve in HPV-induced proliferation, which enriches our understanding of lncRNAs and coding transcripts anticipated in HPV oncogenesis of cervical cancer.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 10232