Piezo2 protein: A novel regulator of tumor angiogenesis and hyperpermeability
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Hong Yang1,2,*, Chang Liu1,2,*, Rong-Mei Zhou1,2,*, Jin Yao1,2,*, Xiu-Miao Li1, Yi Shen1, Hong Cheng3, Jun Yuan4, Biao Yan2, Qin Jiang1,2
1Eye Hospital, Nanjing Medical University, Nanjing, China
2The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China
3Department of Neurology, Jiangsu Province Hospital, Nanjing, China
4Department of Neurology, Jiangsu Chinese Medicine Hospital, Nanjing, China
*These authors have contributed equally to this work
Qin Jiang, email: [email protected]
Biao Yan, email: [email protected]
Keywords: tumor angiogenesis, Piezo2, vascular permeability, endothelial cell
Received: February 29, 2016 Accepted: May 29, 2016 Published: June 17, 2016
Angiogenesis is important for invasive tumor growth and metastasis. Its inhibition is a promising tactic for limiting tumor progression. Here, we showed that Piezo2 knockdown led to decreased glioma angiogenesis and reduced vascular hyperpermeability. Piezo2 was highly expressed in tumor endothelial cells, and its knockdown suppressed vascular leakage and tumor angiogenesis. In a retinal vasculature development assay, corneal angiogenesis assay and a modified Miles assay, Piezo2 knockdown obviously decreased angiogenesis and vascular hyperpermeability. In vitro assays revealed that Piezo2 knockdown inhibited endothelial cell proliferation, migration, and tube formation. Moreover, In vitro co-culture system assay showed that Piezo2 knockdown in endothelial cells suppressed cell proliferation, migration, and invasion of glioma tumor cells. Piezo2 could regulate glioma angiogenesis via Ca2+/Wnt11/β-catenin signaling in endothelial cells. Taken together, these studies provide the evidence for Piezo2 as a critical regulator of tumor angiogenesis and vascular permeability.
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