Oncotarget

Research Papers:

Hypoxia-mediated upregulation of MCT1 expression supports the glycolytic phenotype of glioblastomas

Vera Miranda-Gonçalves, Sara Granja, Olga Martinho, Mrinalini Honavar, Marta Pojo, Bruno M. Costa, Manuel M. Pires, Célia Pinheiro, Michelle Cordeiro, Gil Bebiano, Paulo Costa, Rui M. Reis and Fátima Baltazar _

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Oncotarget. 2016; 7:46335-46353. https://doi.org/10.18632/oncotarget.10114

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Abstract

Vera Miranda-Gonçalves1,2, Sara Granja1,2, Olga Martinho1,2,3, Mrinalini Honavar4, Marta Pojo1,2, Bruno M. Costa1,2, Manuel M. Pires5, Célia Pinheiro6, Michelle Cordeiro7, Gil Bebiano7, Paulo Costa8, Rui M. Reis1,2,3, Fátima Baltazar1,2

1Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal

2ICVS/3B’s-PT Government Associate Laboratory, Braga/Guimarães, Portugal

3Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, São Paulo, Brazil

4Department of Pathology, Hospital Pedro Hispano, Matosinhos, Portugal

5Unit of Neuropathology, Centro Hospitalar do Porto, Porto, Portugal

6Department of Neurosurgery, Centro Hospitalar do Porto, Porto, Portugal

7Hospital Dr. Nélio Mendonça, Funchal, Madeira, Portugal

8Radiotherapy Service, Centro Hospitalar do Montijo, Setúbal, Portugal

Correspondence to:

Fátima Baltazar, email: fbaltazar@ecsaude.uminho.pt

Rui M. Reis, email: ruireis.hcb@gmail.com

Keywords: monocarboxylate transporters (MCTs), tumor hypoxia, lactate, glioblastomas, Warburg effect

Received: February 04, 2016    Accepted: June 02, 2016    Published: June 16, 2016

ABSTRACT

Background: Glioblastomas (GBM) present a high cellular heterogeneity with conspicuous necrotic regions associated with hypoxia, which is related to tumor aggressiveness. GBM tumors exhibit high glycolytic metabolism with increased lactate production that is extruded to the tumor microenvironment through monocarboxylate transporters (MCTs). While hypoxia-mediated regulation of MCT4 has been characterized, the role of MCT1 is still controversial. Thus, we aimed to understand the role of hypoxia in the regulation of MCT expression and function in GBM, MCT1 in particular.

Methods: Expression of hypoxia- and glycolytic-related markers, as well as MCT1 and MCT4 isoforms was assessed in in vitro and in vivo orthotopic glioma models, and also in human GBM tissues by immunofluorescence/immunohistochemistry and Western blot. Following MCT1 inhibition, either pharmacologically with CHC (α-cyano-4-hydroxynnamic acid) or genetically with siRNAs, we assessed GBM cell viability, proliferation, metabolism, migration and invasion, under normoxia and hypoxia conditions.

Results: Hypoxia induced an increase in MCT1 plasma membrane expression in glioma cells, both in in vitro and in vivo models. Additionally, treatment with CHC and downregulation of MCT1 in glioma cells decreased lactate production, cell proliferation and invasion under hypoxia. Moreover, in the in vivo orthotopic model and in human GBM tissues, there was extensive co-expression of MCT1, but not MCT4, with the GBM hypoxia marker CAIX.

Conclusion: Hypoxia-induced MCT1 supports GBM glycolytic phenotype, being responsible for lactate efflux and an important mediator of cell survival and aggressiveness. Therefore, MCT1 constitutes a promising therapeutic target in GBM.


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