Laminin-111 peptide C16 regulates invadopodia activity of malignant cells through β1 integrin, Src and ERK 1/2
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Adriane S. Siqueira1,6, Monique P. Pinto1, Mário C. Cruz2, Basilio Smuczek1, Karen S.P. Cruz3, José Alexandre M. Barbuto3, Daisuke Hoshino4, Alissa M. Weaver5, Vanessa M. Freitas1, Ruy G. Jaeger1
1Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, 05508-000, Brazil
2ICB Core Facility, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, 05508-000, Brazil
3Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, 05508-000, Brazil
4Division of Cancer Cell Research, Kanagawa Cancer Center, Yokohama, Kanagawa, 241-8515, Japan
5Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA
6School of Dentistry, Positivo University, Curitiba, PR, 81280-330, Brazil
Ruy G. Jaeger, email: firstname.lastname@example.org
Keywords: laminin, invadopodia, β1 integrin, ERK 1-2 pathway, Src kinases
Received: October 08, 2015 Accepted: June 04, 2016 Published: June 15, 2016
Laminin peptides influence tumor behavior. In this study, we addressed whether laminin peptide C16 (KAFDITYVRLKF, γ1 chain) would increase invadopodia activity of cells from squamous cell carcinoma (CAL27) and fibrosarcoma (HT1080). We found that C16 stimulates invadopodia activity over time in both cell lines. Rhodamine-conjugated C16 decorates the edge of cells, suggesting a possible binding to membrane receptors. Flow cytometry showed that C16 increases activated β1 integrin, and β1 integrin miRNA-mediated depletion diminishes C16-induced invadopodia activity in both cell lines. C16 stimulates Src and ERK 1/2 phosphorylation, and ERK 1/2 inhibition decreases peptide-induced invadopodia activity. C16 also increases cortactin phosphorylation in both cells lines. Based on our findings, we propose that C16 regulates invadopodia activity over time of squamous carcinoma and fibrosarcoma cells, probably through β1 integrin, Src and ERK 1/2 signaling pathways.
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