Oncotarget

Research Papers:

Ginsenoside Rg1 and platelet-rich fibrin enhance human breast adipose-derived stem cells function for soft tissue regeneration

Fang-Tian Xu, Zhi-Jie Liang, Hong-Mian Li, Qi-Liu Peng, Min-Hong Huang, De-Quan Li, Yi-Dan Liang, Gang-Yi Chi, De-Hui Li, Bing-Chao Yu and Ji-Rong Huang _

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Oncotarget. 2016; 7:35390-35403. https://doi.org/10.18632/oncotarget.9360

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Abstract

Fang-Tian Xu1,*, Zhi-Jie Liang2,*, Hong-Mian Li3, Qi-Liu Peng4, Min-Hong Huang2, De-Quan Li5, Yi-Dan Liang4, Gang-Yi Chi3, De-Hui Li6, Bing-Chao Yu6, Ji-Rong Huang6

1Department of Orthopedics, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China

2Department of Hepatobiliary and Gland Surgery, The Fifth Affiliated Hospital of Guangxi Medical University & The First People’s Hospital of Nanning, Nanning 530022, China

3Department of Plastic and Aesthetic Surgery, The Fifth Affiliated Hospital of Guangxi Medical University & The First People’s Hospital of Nanning, Nanning 530022, China

4Central Laboratory of Medical Science, The Fifth Affiliated Hospital of Guangxi Medical University & The First People’s Hospital of Nanning, Nanning 530022, China

5Department of Breast Surgery, The Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China

6Department of Burns and Plastic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

*These authors have contributed equally to this work as co-first authors

Correspondence to:

Hong-Mian Li, e-mail: doctorlee2228@163.com

Keywords: human breast adipose-derived stem cells, collagen type I sponge scaffolds, ginsenoside Rg1, platelet rich fibrin, soft tissue regeneration

Received: November 20, 2015     Accepted: April 11, 2016     Published: May 14, 2016

ABSTRACT

Adipose-derived stem cells (ASCs) can be used to repair soft tissue defects, wounds, burns, and scars and to regenerate various damaged tissues. The cell differentiation capacity of ASCs is crucial for engineered adipose tissue regeneration in reconstructive and plastic surgery. We previously reported that ginsenoside Rg1 (G-Rg1 or Rg1) promotes proliferation and differentiation of ASCs in vitro and in vivio. Here we show that both G-Rg1 and platelet-rich fibrin (PRF) improve the proliferation, differentiation, and soft tissue regeneration capacity of human breast adipose-derived stem cells (HBASCs) on collagen type I sponge scaffolds in vitro and in vivo. Three months after transplantation, tissue wet weight, adipocyte number, intracellular lipid, microvessel density, and gene and protein expression of VEGF, HIF-1α, and PPARγ were higher in both G-Rg1- and PRF-treated HBASCs than in control grafts. More extensive new adipose tissue formation was evident after treatment with G-Rg1 or PRF. In summary, G-Rg1 and/or PRF co-administration improves the function of HBASCs for soft tissue regeneration engineering.


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