Research Papers: Gerotarget (Focus on Aging):
Epigallocatechin gallate affects glucose metabolism and increases fitness and lifespan in Drosophila melanogaster
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Anika E. Wagner1, Stefanie Piegholdt1, Doerte Rabe1, Nieves Baenas1,2, Anke Schloesser1, Manfred Eggersdorfer3, Achim Stocker4, Gerald Rimbach1
1Institute of Human Nutrition and Food Science, University of Kiel, Kiel, Germany
2Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, Murcia, Spain
3DSM Nutritional Products, Basel, Switzerland
4Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland
Anika E. Wagner, e-mail: firstname.lastname@example.org
Gerald Rimbach, e-mail: email@example.com
Keywords: Gerotarget, epigallocathechin-3-gallate, green tea, life span, Drosophila melanogaster
Received: July 07, 2015 Accepted: August 28, 2015 Published: September 08, 2015
In this study, we tested whether a standardized epigallocatechin-3-gallate (EGCG) rich green tea extract (comprising > 90% EGCG) affects fitness and lifespan as well as parameters of glucose metabolism and energy homeostasis in the fruit fly, Drosophila melanogaster. Following the application of the green tea extract a significant increase in the mean lifespan (+ 3.3 days) and the 50% survival (+ 4.3 days) as well as improved fitness was detected. These effects went along an increased expression of Spargel, the homolog of mammalian PGC1α, which has been reported to affect lifespan in flies. Intriguingly, in flies, treatment with the green tea extract decreased glucose concentrations, which were accompanied by an inhibition of α-amylase and α-glucosidase activity. Computational docking analysis proved the potential of EGCG to dock into the substrate binding pocket of α-amylase and to a greater extent into α-glucosidase. Furthermore, we demonstrate that EGCG downregulates insulin-like peptide 5 and phosphoenolpyruvate carboxykinase, major regulators of glucose metabolism, as well as the Drosophila homolog of leptin, unpaired 2. We propose that a decrease in glucose metabolism in connection with an upregulated expression of Spargel contribute to the better fitness and the extended lifespan in EGCG-treated flies.
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