The stromal genome heterogeneity between breast and prostate tumors revealed by a comparative transcriptomic analysis
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Kan He1,*, Wenwen Lv1,*, Dongni Zheng1,*, Fei Cheng1,*, Tao Zhou1, Shoudong Ye1,2,3, Qian Ban1, Qilong Ying2, Bei Huang1, Lei Chen1, Guohua Wu4 and Dahai Liu1
1 Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei City, Anhui, China
2 Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA
3 Department of Molecular Genetics, Shanghai Medical School, Fudan University, Shanghai, China
4 Laboratory of Quality & Safety Risk Assessment for Sericultural Products and Edible Insects, Ministry of Agriculture, College of Biotechnology and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, China
* These authors have contributed equally to this work
Kan He, email:
Dahai Liu, email:
Keywords: heterogeneity, breast tumor, prostate tumor, GSEA, pathway
Received: January 12, 2015 Accepted: February 12, 2015 Published: March 04, 2015
Stromal microenvironment increases tumor cell survival, proliferation and migration, and promotes angiogenesis. In order to provide comprehensive information on the stromal heterogeneity of diverse tumors, here we employed the microarray datasets of human invasive breast and prostate cancer-associated stromals and applied Gene Set Enrichment Analysis (GSEA) to compare the gene expression profiles between them. As a result, 8 up-regulated pathways and 73 down-regulated pathways were identified in the breast tumor stroma, while 32 up-regulated pathways and 18 down-regulated pathways were identified in the prostate tumor stroma. Only 9 pathways such as tryptophan metabolism were commonly up or down regulated, but most of them (including ABC transporters) were specific for these two tumors. Several essential tumors stromal marker genes were also significantly identified. For example, CDH3 was significantly up-regulated in the stromals of both breast and prostate tumors, however EGFR was only significantly down-regulated in the stromal of breast tumor. Our study would be helpful for future therapeutic and predictive applications in breast and prostate cancers.
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