Oncotarget

Research Papers:

Metabolomic alterations in invasive ductal carcinoma of breast: A comprehensive metabolomic study using tissue and serum samples

Tushar H. More, Sourav RoyChoudhury, Joel Christie, Khushman Taunk, Anupama Mane, Manas K. Santra, Koel Chaudhury and Srikanth Rapole _

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Oncotarget. 2018; 9:2678-2696. https://doi.org/10.18632/oncotarget.23626

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Abstract

Tushar H. More1,2, Sourav RoyChoudhury3, Joel Christie1, Khushman Taunk1, Anupama Mane4, Manas K. Santra5, Koel Chaudhury3 and Srikanth Rapole1

1Proteomics Lab, National Center for Cell Science, Ganeshkhind, Pune 411007, MH, India

2Savitribai Phule Pune University, Ganeshkhind, Pune 411007, MH, India

3School of Medical Science and Technology, Indian Institute of Technology, Kharagpur 721302, WB, India

4Grant Medical Foundation, Ruby Hall Clinic, Pune 411001, MH, India

5Cancer Biology and Epigenetics Lab, National Center for Cell Science, Ganeshkhind, Pune 411007, MH, India

Correspondence to:

Srikanth Rapole, email: rsrikanth@nccs.res.in

Keywords: Invasive ductal carcinoma; targeted metabolomics; untargeted metabolomics; tissue; serum

Received: May 18, 2017     Accepted: December 08, 2017     Published: December 23, 2017

ABSTRACT

Invasive ductal carcinoma (IDC) is the most common type of breast cancer and the leading cause of breast cancer related mortality. In the present study, metabolomic profiles of 72 tissue samples and 146 serum samples were analysed using targeted liquid chromatography multiple reaction monitoring mass spectrometry (LC-MRM/MS) and untargeted gas chromatography mass spectrometry (GC-MS) approaches. Combination of univariate and multivariate statistical treatment identified significant alterations of 42 and 32 metabolites in tissue and serum samples of IDC, respectively when compared to control. Some of the metabolite changes from tissue were also reflected in serum, indicating a bi-directional interaction of metabolites in IDC. Additionally, 8 tissue metabolites and 9 serum metabolites showed progressive change from control to benign to IDC suggesting their possible role in malignant transformation. We have identified a panel of three metabolites viz. tryptophan, tyrosine, and creatine in tissue and serum, which could be useful in screening of IDC subjects from both control and benign. The metabolomic alterations in IDC showed perturbations in purine and pyrimidine metabolism, amino sugar metabolism, amino acid metabolism, fatty acid biosynthesis etc. Comprehensively, this study provides valuable insights into metabolic adaptations of IDC, which can help to identify diagnostic markers as well as potential therapeutic targets.


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