Oncotarget

Research Papers:

Spatial function of the oxidative DNA damage response in radiation induced bystander effects in intra- and inter-system of Caenorhabditis elegans

Qingqing Li, Jue Shi, Lianyun Chen, Furu Zhan, Hang Yuan, Jun Wang, An Xu and Lijun Wu _

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Oncotarget. 2017; 8:51253-51263. https://doi.org/10.18632/oncotarget.17229

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Abstract

Qingqing Li1,2, Jue Shi1,2, Lianyun Chen1, Furu Zhan1, Hang Yuan1, Jun Wang1, An Xu1 and Lijun Wu1,2,3

1Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, P. R. China

2University of Science and Technology of China, Hefei, Anhui 230026, P. R. China

3Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province, Hefei, Anhui 230031, P. R. China

Correspondence to:

Lijun Wu, email: ljw@ipp.ac.cn

Keywords: C. elegans, DDR, tissue specific RNAi, ROS, RIBE

Abbreviations: RIBE, radiation induced bystander effects; DDR, DNA damage response; ROS, reactive oxygen species; NGM, nematode growth medium

Received: February 15, 2017    Accepted: March 24, 2017    Published: April 19, 2017

ABSTRACT

Though the signaling events involved in radiation induced bystander effects (RIBE) have been investigated both in vitro and in vivo, the spatial function of these communications, especially the related signaling pathways, is not fully elucidated. In the current study, significant increases of DNA damage were clearly observed in C. elegans germline upon irradiation to both intra-system of posterior pharynx and inter-system of vulva, in which more severe damage, even to F1 generation worms, was shown for vulva irradiation. Spatial function assay indicated the DDR key components of mrt-2/hus-1/cep-1/ced-4 were indispensable in germ cells for both sites irradiation, while those components in somatic cells were either not (cep-1/ced-4) or partially (mrt-2/hus-1) required to promote apoptosis. Moreover, production of reactive oxygen species (ROS) indicated by the superoxide dismutase expression and the unfolded protein response of the mitochondria was found systemically involved in the initiation of these processes for both two site irradiation. These results will give a better understanding of the RIBE mechanisms in vivo, and invaluable to assess the clinical relevance to radiotherapy.


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