Identification and characterization of L1-specific endo-siRNAs essential for early embryonic development in pig

Heng Zhang; Jilong Liu; Yurong Tai; Xiaolei Zhang; Jiaming Zhang; Shichao Liu; Jiawei Lv; Zhonghua Liu; Qingran Kong

doi:10.18632/oncotarget.15517

Oncotarget

Oncotarget (a primarily oncology-focused, peer-reviewed, open access journal) aims to maximize research impact through insightful peer-review; eliminate borders between specialties by linking different fields of oncology, cancer research and biomedical sciences; and foster application of basic and clinical science.

Its scope is unique. The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. The term was introduced in the inaugural Editorial, Introducing Oncotarget.

As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. Papers will now be published continuously within yearly volumes in their final and complete form and then quickly released to Pubmed.

Subscribe to receive alerts once a paper has been published by Oncotarget.

Impact Journals, LLC is the publisher of Oncotarget: www.impactjournals.com.

Impact Journals is a member of the Wellcome Trust List of Compliant Publishers.

Impact Journals is a member of the Society for Scholarly Publishing.

On December 23, 2022, Oncotarget server experienced a DDoS attack. As a result, Oncotarget site was inaccessible for a few hours. Oncotarget team swiftly dealt with the situation and took it under control. This malicious action will be reported to the FBI.

Research Papers:

Identification and characterization of L1-specific endo-siRNAs essential for early embryonic development in pig

Heng Zhang, Jilong Liu, Yurong Tai, Xiaolei Zhang, Jiaming Zhang, Shichao Liu, Jiawei Lv, Zhonghua Liu and Qingran Kong _

PDF | HTML | Supplementary Files | How to cite

Oncotarget. 2017; 8:23167-23176. https://doi.org/10.18632/oncotarget.15517

Metrics: PDF 1630 views | HTML 1978 views | ?

Abstract

Heng Zhang1,*, Jilong Liu2,*, Yurong Tai1,*, Xiaolei Zhang1, Jiaming Zhang1, Shichao Liu1, Jiawei Lv1, Zhonghua Liu1, Qingran Kong1

1Laboratory of Embryo Biotechnology, College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, China

2College of Veterinary Medicine, South China Agricultural University, Guangzhou Province 510642, China

*These authors contributed equally to this work

Correspondence to:

Qingran Kong, email: [email protected]

Zhonghua Liu, email: [email protected]

Keywords: endo-siRNA, deep sequencing, L1, early embryonic development, pig

Received: December 15, 2016 Accepted: February 08, 2017 Published: February 19, 2017

ABSTRACT

Small noncoding RNAs (sncRNAs) play important roles in RNA interference (RNAi). In addition to microRNA (miRNA) and Piwi-interacting RNA (piRNA), one key member of sncRNAs group is endogenous small interfering RNA (endo-siRNA). Some studies do show the role of endo-siRNAs in Dicer and/or Ago mutants, however, the biological functions of specific endo-siRNAs remains mostly unanswered. In the study, we have performed a comparative analysis of endo-siRNAs present in porcine sperms, oocytes and zygotes, identified by deep sequencing and bioinformatics analysis. Further, we observe a large amount of endo-siRNAs specific binding on ORF2 and 3′ UTR of porcine L1 (L1-siRNAs). And, 9 L1-siRNAs generated from a dsRNA formed between L1 transcript and a newly identified an antisense noncoding RNA was characterized. We show the L1-siRNAs regulate early embryonic development by inhibiting the activity of L1 retrotransposition. This work can contribute to understanding the functional role of abundant endo-siRNAs in embryonic development.

All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 15517

Publication Alerts

Research Papers:

Identification and characterization of L1-specific endo-siRNAs essential for early embryonic development in pig

Abstract