Oncotarget

Research Papers:

ABCG2 confers promotion in gastric cancer through modulating downstream CRKL in vitro combining with biostatistics mining

Junqing Wang _, Zhou Yunyun, Lu Wang, Xuehua Chen and Zhenggang Zhu

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Oncotarget. 2017; 8:5256-5267. https://doi.org/10.18632/oncotarget.14128

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Abstract

Junqing Wang1,2,3, Zhou Yunyun4, Lu Wang5, Xuehua Chen2,3, Zhenggang Zhu1,2,3

1Department of Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 20025, People’s Republic of China

2Shanghai Key Laboratory of Gastric Neoplasms, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 20025, People’s Republic of China

3Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 20025, People’s Republic of China

4Department of Data Science, University of Mississippi Medical Center, Jackson, MS 39216, USA

5McArdle Laboratory for Cancer Research, University of Wisconsin School of Medicine and Public Health, Madison, WI, 53706, USA

Correspondence to:

Junqing Wang, email: wangjunqingmd@hotmail.com

Zhenggang Zhu, email: scienceused_labrjh@yeah.com

Keywords: ABCG2, CRKL, cell proliferation, cell apoptosis, gastric cancer

Received: September 20, 2016    Accepted: November 23, 2016    Published: December 23, 2016

ABSTRACT

ABCG2, member of ATP-binding cassette (ABC) transporter family, is known as crucial regulator related to multi-drug resistance in human tumors and has recently been putatively studied as human carcinoma cell biomarker. While, effects of ABCG2 on human gastric cancer (GC) has not been illustrated thoroughly. In this study, by applying biostatistics mining methods, we observed that ABCG2 is frequently aberrantly expressed in GC patients through exploring dataset of GSE19826 in NCBI GEO database. Contemporary, extreme up-regulation of ABCG2 was discovered in both GC specimens and cell lines of our center, from which we observed high level of ABCG2 associated with GC clinicopathologic features and poor outcomes. Depletion of ABCG2 in MKN-45 GC cells, the cell proliferation was significantly impacted along with cell cycle arrest, and cell apoptosis was induced. Interestingly, combined with data mining of NCBI database, CRKL, a pivotal GC promoter, presents a significant positive correlation with ABCG2. And the expression of CRKL in GC cells was obviously affected through ABCG2 depletion. Simultaneously, over-expression of CRKL in MKN-45 cells significantly rescued most of the phenotypes induced by ABCG2 depletion. Thus, we suggest that ABCG2 is a potential biomarker and target upstream CRKL, which could be further studied for GC diagnosis and therapeutic treatment


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