Oncotarget

Research Papers: Pathology:

Activation of volume-sensitive Cl- channel mediates autophagy-related cell death in myocardial ischaemia/reperfusion injury

Yuesheng Xia, Yan Liu, Tong Xia, Xing Li, Cong Huo, Xin Jia, Lin Wang, Rong Xu, Ning Wang, Mingming Zhang, Hong Li and Xiaoming Wang _

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Oncotarget. 2016; 7:39345-39362. https://doi.org/10.18632/oncotarget.10050

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Abstract

Yuesheng Xia1,*, Yan Liu1,*, Tong Xia2,*, Xing Li1, Cong Huo1, Xin Jia1, Lin Wang1, Rong Xu1, Ning Wang1, Mingming Zhang3, Hong Li1 and Xiaoming Wang1

1 Department of Geriatrics, Xijing Hospital, Fourth Military Medical University, Xi’an, China

2 Department of Preventive Medicine, Sun Yat-sen University, Guangzhou, China

3 Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an, China

* These authors have contributed equally to this work

Correspondence to:

Xiaoming Wang, email:

Keywords: ischemia/reperfusion injury, VSOR Cl- channel, reactive oxygen species, autophagy, cell death, Pathology Section

Received: April 08, 2016 Accepted: June 03, 2016 Published: June 14, 2016

Abstract

Excessive reactive oxygen species (ROS) plays an important role in myocardial ischemia/reperfusion (I/R) injury, which triggers not only myocardial cellular apoptosis but also autophagy-related cell death, in which volume-sensitive outwardly rectifying (VSOR) Cl- channel-activated by ROS contributes to cell apoptotic volume decrease, playing an incipient incident of cellular apoptosis. However, whether VSOR Cl- channel concurrently participates in autophagy-related cell death regulation remains unclear. To illuminate the issue, studies underwent in myocardial vitro and vivo I/R model. Rats were performed to ischemia 30 minutes and subsequent reperfusion 24-96 hours, ROS scavenger (NAC), VSOR Cl- channel blocker (DCPIB) and autophagy inhibitor (3MA) were administered respectively. Results showed that oxidative stress, LC3-II stain and inflammation in myocardial tissue were markedly increased, lysosome associated membrane protein-2 (LAMP2) were significantly reduced with I/R group as compared with sham group, reperfusion significantly led to damage in myocardial tissue and heart function, whereas the disorder could be rescued through these agents. Moreover, primary neonatal rat cardiomyocytes hypoxia/reoxygenation model were administered, results showed that VSOR Cl- channel-activated by reoxygenation could cause both cell volume decrease and intracellular acidification, which further increased LC3 and depleted of LAMP2, resulting in autophagy-related cell death. Interestingly, VSOR Cl- channel-blocked by DCPIB could stably maintain the cell volume, intracellular pH, abundant LAMP2 and autophagic intensity regardless of ROS intension derived from reoxygenation injury or adding H2O2. These results first demonstrate that VSOR Cl- channel-activated is a pivotal event to trigger autophagy-related death, which reveals a novel therapeutic target to decrease myocardial I/R injury.


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