Identification and characterization of novel enhanced cell penetrating peptides for anti-cancer cargo delivery
PDF | HTML | How to cite
Metrics: PDF 1785 views | HTML 2617 views | ?
Xiguang Zhang1, Jean Yves Brossas1,2, Christophe Parizot2, Jean Marc Zini3 and Angelita Rebollo1
1CIMI Paris, Inserm 1135, UPMC, 75013 Paris, France
2Hôpital Pitié Salpêtrière, AP-HP, Department of Immunology, 75013 Paris, France
3Hôpital Saint Louis, AP HP, 75010 Paris, France
Angelita Rebollo, email: [email protected]
Keywords: cell penetrating peptides; peptide internalization; peptide stablity
Received: September 24, 2017 Accepted: November 16, 2017 Published: December 11, 2017
Cell penetrating peptides (CPP) are able cross the membrane and to transport cargos, presenting a great potential in drug delivery and diagnosis. In this paper, we have identified novel natural or synthetic CPPs. We have validated their rapid and efficient time and dose-dependent penetration, the absence of toxicity, the intracellular localization and the stability to proteases degradation, one of the main bottlenecks of peptides. Moreover, we have associate a cargo (an interfering peptide blocking the association of the serine/threonine phosphatase PP2A to its inhibitor, the oncogene SET) to the new generated shuttles and showed that they new bi-functional peptides keep the original properties of the shuttle and, in addition, are able to induce apoptosis due to the properties of the cargo. The CPPs identified in this study have promising perspectives for future anti-cancer drug delivery.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.