Silencing DNA methyltransferase 1 (DNMT1) inhibits proliferation, metastasis and invasion in ESCC by suppressing methylation of RASSF1A and DAPK

Jian Bai; Xue Zhang; Kai Hu; Bangqing Liu; Haiyong Wang; Angui Li; Feng Lin; Lifei Zhang; Xiaolin Sun; Zhenzong Du; Jianfei Song

doi:10.18632/oncotarget.9866

Oncotarget

Oncotarget (a primarily oncology-focused, peer-reviewed, open access journal) aims to maximize research impact through insightful peer-review; eliminate borders between specialties by linking different fields of oncology, cancer research and biomedical sciences; and foster application of basic and clinical science.

Its scope is unique. The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. The term was introduced in the inaugural Editorial, Introducing Oncotarget.

As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. Papers will now be published continuously within yearly volumes in their final and complete form and then quickly released to Pubmed.

Subscribe to receive alerts once a paper has been published by Oncotarget.

Impact Journals, LLC is the publisher of Oncotarget: www.impactjournals.com.

Impact Journals is a member of the Wellcome Trust List of Compliant Publishers.

Impact Journals is a member of the Society for Scholarly Publishing.

On December 23, 2022, Oncotarget server experienced a DDoS attack. As a result, Oncotarget site was inaccessible for a few hours. Oncotarget team swiftly dealt with the situation and took it under control. This malicious action will be reported to the FBI.

Research Papers:

Silencing DNA methyltransferase 1 (DNMT1) inhibits proliferation, metastasis and invasion in ESCC by suppressing methylation of RASSF1A and DAPK

Jian Bai _, Xue Zhang, Kai Hu, Bangqing Liu, Haiyong Wang, Angui Li, Feng Lin, Lifei Zhang, Xiaolin Sun, Zhenzong Du and Jianfei Song

PDF | HTML | Supplementary Files | How to cite

Oncotarget. 2016; 7:44129-44141. https://doi.org/10.18632/oncotarget.9866

Metrics: PDF 2150 views | HTML 2424 views | ?

Abstract

Jian Bai1, Xue Zhang2, Kai Hu3, Bangqing Liu1, Haiyong Wang3, Angui Li3, Feng Lin3, Lifei Zhang1, Xiaolin Sun3, Zhenzong Du1,4, Jianfei Song1,4

1Department of Thoracic & Cardiovascular Surgery, The Second Affiliated Hospital of Guilin Medical University, Guilin, China

2Department of ICU, The Second Affiliated Hospital of Guilin Medical University, Guilin, China

3Department of Thoracic & Cardiovascular Surgery, The Affiliated Hospital of Guilin Medical University, Guilin, China

4Current address: Department of Thoracic & Cardiovascular Surgery, The Second Affiliated Hospital of Guilin Medical University, Lingui District, Guilin, China

Correspondence to:

Jianfei Song, email: [email protected]

Zhenzong Du, email: [email protected]

Keywords: ESCC, DNA methyltransferase 1, methylation, RASSF1A, DAPK

Received: July 27, 2015 Accepted: March 31, 2016 Published: June 7, 2016

ABSTRACT

Our previous study showed DNMT1 is up-regulated in esophageal squamous cell carcinoma (ESCC), which is associated with methylation of tumor suppressors. In the current study, we investigate the role of DNMT1 in ESCC. We found silencing DNMT1 inhibited proliferation, metastasis and invasion of three different ESCC cells, K150, K410 and K450. We also found silencing DNMT1 induced G1 arrest and cell apoptosis in K150, K410 and K450 cells. In vivo study showed silencing DNMT1 suppressed tumor growth in nude mice. In addition, silencing DNMT1 increased expression of tumor suppressor genes, RASSF1A and DAPK, in ESCC cells and ESCC xenograft in nude mice. Moreover, silencing DNMT1 decreased methylation in promoter of RASSF1A and DAPK. In conclusion, our data demonstrated that silencing DNMT1 inhibits proliferation, metastasis and invasion in ESCC by suppressing methylation of RASSF1A and DAPK.

All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 9866

Publication Alerts

Research Papers:

Silencing DNA methyltransferase 1 (DNMT1) inhibits proliferation, metastasis and invasion in ESCC by suppressing methylation of RASSF1A and DAPK

Abstract