Research Papers:
The effects of blueberry anthocyanins on histone acetylation in rat liver fibrosis
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Abstract
Wei Zhan1,*, Xin Liao2,*, Ru-Jia Xie3,*, Tian Tian3, Lei Yu3, Xing Liu3, Jing Liu2, Po Li4, Bing Han3, Ting Yang3, Bei Zhang5, Li-Jun Cai6, Rui Li6 and Qin Yang3
1General Surgery of The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China
2Imaging Department of The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China
3Department of Physiology of The Guizhou Medical University, Guiyang 550004, Guizhou Province, China
4Department of Pathology of The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China
5Ultrasonic Center of The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China
6Department of Neurology of The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China
7Department of Recovery of the Guizhou People’s Hospital, Guiyang 550004, Guizhou Province, China
*Co-first authors
Correspondence to:
Bing Han, email: [email protected]
Qin Yang, email: [email protected]
Keywords: blueberry, anthocyanin, hepatic stellate cell, liver fibrosis, histone acetylation
Received: January 18, 2017 Accepted: April 17, 2017 Published: May 12, 2017
ABSTRACT
To determine the effects ofanthocyanins from blueberries on hepatic stellate cell (HSCs-T6) and on histone acetylation during liver fibrosis induced by CCl4 in rats. Fifty male SD rats weighing 180 ± 20g were randomly placed into a control group, a hepatic fibrosis group, a blueberry treatment group, a blueberry intervention group, and a natural recovery group. After the rats were sacrificed, the livers and the liver indexes were measured, and the pathological changes were observed by HE staining and Masson staining. The blood was analyzed for the four indexes of liver fibrosis and liver function; nucleoprotein from liver tissues and karyoplasm were isolated to determine the expression of acH3K9, acH3K14, and acH3K18 by Western blotting.
Compared with the lethal rate of the control group, the median lethal rate of HSCs-T6 cells treated with a the 50μmol/L concentration was 66.94% (P < 0.05). The protein expression on α-SMA, type I collagen, TIMP1 significantly decreased (P < 0.05) following treatment with 50 ug/ml of anthocyanin for 36 h; moreover, the expression of acH3K9, acH3K14 and acH3K18 modification were up-regulated (P < 0.05). Furthermore, compared with the liver in the model group, the liver in the intervention group showed the most obvious improvement (P < 0.01), and its karyoplasm had increased expression of acH3K9, acH3K14 and acH3K18 (P<0.01).
Regulating histone acetylation could improve liver function and liver fibrosis indexes in rats with hepatic fibrosis. The mechanism might be related to certain genes that promote apoptosis, so as to inhibit the effect of anti hepatic fibrosis.
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