Oncotarget

Research Papers:

MiR-124-3p attenuates hyperphosphorylation of Tau protein-induced apoptosis via caveolin-1-PI3K/Akt/GSK3β pathway in N2a/APP695swe cells

Qingmei Kang, Yue Xiang, Dan Li, Jie Liang, Xiong Zhang, Fanlin Zhou, Mengyuan Qiao, Yingling Nie, Yurong He, Jingyi Cheng, Yubing Dai and Yu Li _

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Oncotarget. 2017; 8:24314-24326. https://doi.org/10.18632/oncotarget.15149

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Abstract

Qingmei Kang1,2,*, Yue Xiang1,2,*, Dan Li1,2, Jie Liang1,2, Xiong Zhang2, Fanlin Zhou1,2, Mengyuan Qiao1,2, Yingling Nie1,2, Yurong He1,2, Jingyi Cheng1,2, Yubing Dai3, Yu Li1,2

1Department of Pathology, Chongqing Medical University, Chongqing, 400016, China

2Center for Molecular Medicine Testing, Chongqing Medical University, Chongqing, 400016, China

3Department of Neurosurgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, 550004, China

*These authors contributed equally to this work

Correspondence to:

Yu Li, email: [email protected]

Keywords: miR-124-3p, tau, caveolin-1, PI3K/Akt/GSK3β, Alzheimer's disease

Received: November 02, 2016     Accepted: January 24, 2017     Published: February 07, 2017

ABSTRACT

Hyperphosphorylation of Tau forming neurofibrillary tangles has been considered as a crucial event in the pathogenesis of Alzheimer's disease (AD). MiR-124-3p belongs to microRNA (miRNA) family and was markedly decreased in AD, however, the functions of miR-124-3p in the pathogenesis of AD remain unknown. We observed that the expression of miR-124-3p was significantly decreased in N2a/APP695swe cells; and transfection of miR-124-3p mimics not only attenuated cell apoptosis and abnormal hyperphosphorylation of Tau protein without any changes of total Tau protein, but also increased expression levels of Caveolin-1, phosphoinositide 3-kinase (PI3K), phospho-Akt (Akt-Ser473)/Akt, phospho-glycogen synthase kinase-3 beta (GSK-3β-Ser9)/GSK-3β in N2a/APP695swe cells. We further found that miR-12-3p directly targeted Caveolin-1; miR-124-3p inhibited abnormal hyperphosphorylation of Tau by regulating Caveolin-1-PI3K/Akt/GSK3β pathway in AD. This study reveals that miR-124-3p may play a neuroprotective role in AD, which may provide new ideas and therapeutic targets for AD.


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