Resveratrol induces mitochondria-mediated, caspase-independent apoptosis in murine prostate cancer cells

Sanjay Kumar; Erdal Eroglu; James A. Stokes III; Karyn Scissum-Gunn; Sabita N. Saldanha; Udai P. Singh; Upender Manne; Selvarangan Ponnazhagan; Manoj K. Mishra

doi:10.18632/oncotarget.14947

Oncotarget

Oncotarget (a primarily oncology-focused, peer-reviewed, open access journal) aims to maximize research impact through insightful peer-review; eliminate borders between specialties by linking different fields of oncology, cancer research and biomedical sciences; and foster application of basic and clinical science.

Its scope is unique. The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. The term was introduced in the inaugural Editorial, Introducing Oncotarget.

As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. Papers will now be published continuously within yearly volumes in their final and complete form and then quickly released to Pubmed.

Subscribe to receive alerts once a paper has been published by Oncotarget.

Impact Journals, LLC is the publisher of Oncotarget: www.impactjournals.com.

Impact Journals is a member of the Wellcome Trust List of Compliant Publishers.

Impact Journals is a member of the Society for Scholarly Publishing.

On December 23, 2022, Oncotarget server experienced a DDoS attack. As a result, Oncotarget site was inaccessible for a few hours. Oncotarget team swiftly dealt with the situation and took it under control. This malicious action will be reported to the FBI.

Research Papers:

Resveratrol induces mitochondria-mediated, caspase-independent apoptosis in murine prostate cancer cells

Sanjay Kumar, Erdal Eroglu, James A. Stokes III, Karyn Scissum-Gunn, Sabita N. Saldanha, Udai P. Singh, Upender Manne, Selvarangan Ponnazhagan and Manoj K. Mishra _

PDF | HTML | Supplementary Files | How to cite

Oncotarget. 2017; 8:20895-20908. https://doi.org/10.18632/oncotarget.14947

Metrics: PDF 2004 views | HTML 30022 views | ?

Abstract

Sanjay Kumar1, Erdal Eroglu2, James A. Stokes III1, Karyn Scissum-Gunn1, Sabita N. Saldanha1, Udai P. Singh3, Upender Manne4, Selvarangan Ponnazhagan4, Manoj K. Mishra1

1Cancer Biology Research and Training, Department of Biological Sciences, Alabama State University, Montgomery, AL, USA

2Faculty of Engineering, Bioengineering Department, Celal Bayar University, Muradiye, Manisa, Turkey

3Department of Pathology, Microbiology and Immunology, University of South Carolina, Columbia, SC, USA

4Department of Pathology, School of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA

Correspondence to:

Manoj K. Mishra, email: [email protected]

Keywords: apoptosis, mitochondria, prostate cancer cells, resveratrol

Received: March 06, 2016 Accepted: January 10, 2017 Published: February 01, 2017

ABSTRACT

Found in the skins of red fruits, including grapes, resveratrol (RES) is a polyphenolic compound with cancer chemopreventive activity. Because of this activity, it has gained interest for scientific investigations. RES inhibits tumor growth and progression by targeting mitochondria-dependent or -independent pathways. However, further investigations are needed to explore the underlying mechanisms.

The present study is focused on examining the role of RES-induced, mitochondria-mediated, caspase-independent apoptosis of prostate cancer cells, namely transgenic adenocarcinoma of mouse prostate (TRAMP) cells. These cells were exposed to RES for various times, and cell killing, cell morphology, mitochondrial membrane potential (Δψm), expression of Bax and Bcl2 proteins, the role of caspase-3, and DNA fragmentation were analyzed.

TRAMP cells exposed to RES showed decreased cell viability, altered cell morphology, and disrupted Δψm, which led to aberrant expression of Bax and Bcl2 proteins. Furthermore, since the caspase-3 inhibitor, z-VAD-fmk (benzyloxycarbonyl-valine-alanine-aspartic acid-fluoromethyl ketone), had no appreciable impact on RES-induced cell killing, the killing was evidently caspase-independent. In addition, RES treatment of TRAMP-C1, TRAMP-C2, and TRAMP-C3 cells caused an appreciable breakage of genomic DNA into low-molecular-weight fragments.

These findings show that, in inhibition of proliferation of TRAMP cells, RES induces mitochondria-mediated, caspase-independent apoptosis. Therefore, RES may be utilized as a therapeutic agent to control the proliferation and growth of cancer cells.

All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 14947

Publication Alerts

Research Papers:

Resveratrol induces mitochondria-mediated, caspase-independent apoptosis in murine prostate cancer cells

Abstract