Research Papers:
Genomic characteristics of pancreatic squamous cell carcinoma, an investigation by using high throughput sequencing after in-solution hybrid capture
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Abstract
Meng-Dan Xu1,*, Shu-Ling Liu2,*, Yi-Zhong Feng3,*, Qiang Liu4,*, Meng Shen1, Qiaoming Zhi5, Zeyi Liu6, Dong-Mei Gu7, Jie Yu7, Liu-Mei Shou1,8, Fei-Ran Gong9, Qi Zhu10, Weiming Duan1, Kai Chen1, Junning Zhang2, Meng-Yao Wu1, Min Tao1,11,12,13, Wei Li1,11,12
1Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
2Department of Radiation Oncology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
3Department of Pathology, The Second Affiliated Hospital of Soochow University, Suzhou 215006, China
4Department of Pathology, Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200000, China
5Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
6Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
7Department of Pathology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
8Department of Oncology, The First Affiliated Hospital of Zhejiang Chinese Medicine University, Hangzhou 310006, China
9Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
10Xi'an Tianlong Science and Technology Co., Ltd., Xi'an 710018, China
11PREMED Key Laboratory for Precision Medicine, Soochow University, Suzhou 215021, China
12Jiangsu Institute of Clinical Immunology, Suzhou 215006, China
13Institute of Medical Biotechnology, Soochow University, Suzhou 215021, China
*These authors contributed equally to this work
Correspondence to:
Wei Li, email: [email protected], [email protected]
Min Tao, email: [email protected], [email protected]
Meng-Yao Wu, email: [email protected]
Keywords: pancreatic squamous cell carcinoma, pancreatic adenocarcinoma, high throughput sequencing (HTS), in-solution hybrid capture
Received: October 21, 2016 Accepted: January 09, 2017 Published: January 16, 2017
ABSTRACT
Squamous cell carcinoma (SCC) of pancreas is a rare histotype of pancreatic ductal carcinoma which is distinct from pancreatic adenocarcinoma (AC). Although there are standard treatments for pancreatic AC, no precise therapies exist for pancreatic SCC. Here, we screened 1033 cases of pancreatic cancer and identified 2 cases of pure SCC, which were pathologically diagnosed on the basis of finding definite intercellular bridges and/or focal keratin peal formation in the tumor cells. Immunohistochemistry assay confirmed the positive expression of CK5/6 and p63 in pancreatic SCC. To verify the genomic characteristics of pancreatic SCC, we employed in-solution hybrid capture targeting 137 cancer-related genes accompanied by high throughput sequencing (HTS) to compare the different genetic variants in SCC and AC of pancreas. We compared the genetic alterations of known biomarkers of pancreatic adenocarcinoma in different pancreatic cancer tissues, and identified nine mutated genes in SCC of pancreas: C7orf70, DNHD1, KPRP, MDM4, MUC6, OR51Q1, PTPRD, TCF4, TET2, and nine genes (ABCB1, CSF1R, CYP2C18, FBXW7, ITPA, KIAA0748, SOD2, SULT1A2, ZNF142) that are mutated in pancreatic AC. This study may have taken one step forward on the discovery of potential biomarkers for the targeted treatment of SCC of the pancreas.
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