Oncotarget

Research Papers: Immunology:

Immunoglobulin G promotes skin graft acceptance in an immunologically potent rat model

Xingmu Liu, Tao Huang, Xueling Chen, Meiling Yan, Feiyuan Yu, Huan Gu, Chao He and Jiang Gu _

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Oncotarget. 2016; 7:39408-39420. https://doi.org/10.18632/oncotarget.9823

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Abstract

Xingmu Liu1,2,*, Tao Huang1,*, Xueling Chen1, Meiling Yan1, Feiyuan Yu1, Huan Gu1, Chao He2 and Jiang Gu1

1 Department of Pathology and Provincial Key Laboratory of Infectious Diseases and Immunopathology, Collaborative and Creative Center, Molecular Diagnosis and Personalized Medicine, Shantou University Medical College, Shantou, Guangdong, China

2 Department of General Surgery, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China

* These authors have contributed equally to this work

Correspondence to:

Jiang Gu, email:

Keywords: immunoglobulin G, rat model, transplantation, rejection, cytokines, Immunology and Microbiology Section, Immune response, Immunity

Received: February 25, 2016 Accepted: May 23, 2016 Published: June 05, 2016

Abstract

Immunoglobulin G (IgG) has been shown to protect graft rejection after transplantation, whereas the molecular mechanism of IgG in promoting graft acceptance has not been well established. In this study, we tested the effectiveness of IgG in preventing rejection of transplanted skin graft in an immunologically potent rat model, and studied the mechanism of this protection. We found that systemic or local administration of IgG significantly prolonged the survival of skin grafts with the immune tolerance induced by IgG and subcutaneous local injection of 1mg IgG to adult SD rat yielded the longest survival of skin grafts from 5.8 to 17.3 days. We also found that IgG reduced the number of pro-inflammatory cells especially lymphocytes, neutrophils and basophils, increased the seral levels of anti-inflammatory factors including IL-10 and IL-4, and activated CD4+CD25+Foxp3+ regulatory T cells, unveiling the mechanisms of this protective effect. These findings provide new insight to support clinical application of IgG in treating transplantation.


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