Oncotarget

Research Papers:

miRNA-337-3p inhibits gastric cancer progression through repressing myeloid zinc finger 1-facilitated expression of matrix metalloproteinase 14

Liduan Zheng, Wanju Jiao, Hong Mei, Huajie Song, Dan Li, Xuan Xiang, Yajun Chen, Feng Yang, Huanhuan Li, Kai Huang and Qiangsong Tong _

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Oncotarget. 2016; 7:40314-40328. https://doi.org/10.18632/oncotarget.9739

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Abstract

Liduan Zheng1,2,*, Wanju Jiao1,*, Hong Mei3,*, Huajie Song3, Dan Li3, Xuan Xiang3, Yajun Chen1, Feng Yang3, Huanhuan Li3, Kai Huang2, Qiangsong Tong2,3

1Department of Pathology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, P. R. China

2Clinical Center of Human Genomic Research, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, P. R. China

3Department of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, P. R. China

*These authors contributed equally to this work

Correspondence to:

Qiangsong Tong, email: qs_tong@hotmail.com

Keywords: gastric cancer, microRNA-337-3p, myeloid zinc finger 1, matrix metalloproteinase 14, transcriptional regulation

Received: March 12, 2016     Accepted: May 13, 2016     Published: May 31, 2016

ABSTRACT

Matrix metalloproteinase 14 (MMP-14), a membrane-anchored MMP that promotes the tumorigenesis and aggressiveness, is highly expressed in gastric cancer. However, the transcriptional regulators of MMP-14 expression in gastric cancer still remain largely unknown. In this study, through mining computational algorithm programs and chromatin immunoprecipitation datasets, we identified adjacent binding sites of myeloid zinc finger 1 (MZF1) and miRNA-337-3p (miR-337-3p) within the MMP-14 promoter. We demonstrated that MZF1 directly bound to the MMP-14 promoter to facilitate its nascent transcription and expression in gastric cancer cell lines. In contrast, endogenous miR-337-3p suppressed the MMP-14 expression through recognizing its binding site within MMP-14 promoter. Mechanistically, miR-337-3p repressed the binding of MZF1 to MMP-14 promoter via recruiting Argonaute 2 and inducing repressive chromatin remodeling. Gain- and loss-of-function studies demonstrated that miR-337-3p suppressed the growth, invasion, metastasis, and angiogenesis of gastric cancer cells in vitro and in vivo through repressing MZF1-facilitated MMP-14 expression. In clinical specimens and cell lines of gastric cancer, MZF1 was highly expressed and positively correlated with MMP-14 expression. Meanwhile, miR-337-3p was under-expressed and inversely correlated with MMP-14 levels. miR-337-3p was an independent prognostic factor for favorable outcome of gastric cancer, and patients with high MZF1 or MMP-14 expression had lower survival probability. Taken together, these data indicate that miR-337-3p directly binds to the MMP-14 promoter to repress MZF1-facilitatd MMP-14 expression, thus suppressing the progression of gastric cancer.


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