Tumor-tropic endothelial colony forming cells (ECFCs) loaded with near-infrared sensitive Au nanoparticles: A “cellular stove” approach to the photoablation of melanoma
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Giancarlo Margheri1, Angela Zoppi2,3, Roberto Olmi4, Silvana Trigari1, Rita Traversi5, Mirko Severi5, Daniele Bani6, Francesca Bianchini7, Eugenio Torre7, Francesca Margheri7, Anastasia Chillà7, Alessio Biagioni7, Lido Calorini7,8, Anna Laurenzana7, Gabriella Fibbi7, Mario Del Rosso7,8
1Institute for Complex Systems, National Research Council, Sesto Fiorentino, Italy
2Department of Physics “Enrico Fermi”, University of Pisa, Italy
3Present address: Plasmatech, Department of Physics “Enrico Fermi”, University of Pisa, Pisa, Italy
4Institute of Applied Physics “Nello Carrara”, National Research Council, Sesto Fiorentino, Italy
5Department of Chemistry “Ugo Schiff”, University of Florence, Sesto Fiorentino, Italy
6Department of Clinical and Experimental Medicine, University of Florence, Florence, Italy
7Department of Experimental and Clinical Biomedical Science, University of Florence, Florence, Italy
8Excellence Center for Research, Transfer and High Education ‘Study at Molecular and Clinical Level of Chronic, Inflammatory, Degenerative and Neoplastic Disorders for the Development on Novel Therapies’, Florence, Italy
Anna Laurenzana, email: firstname.lastname@example.org
Gabriella Fibbi, email: email@example.com
Keywords: gold nanoparticles, endothelial colony forming cells (ECFCs), chemokine (C-X-C motif) receptor 4 (CXCR4), melanoma, tumor thermoablation
Received: January 13, 2016 Accepted: May 08, 2016 Published: May 20, 2016
In the photothermal treatments (PTs) of tumor, the localization of a high number of near-infrared (NIR) absorbing gold nanoparticles in the tumor mass is still a challenging issue. Here, we propose a promising strategy to deliver therapeutic chitosan-coated gold nanoparticles to tumor cells as hidden cargo of Endothelial Colony Forming Cells (ECFCs) endowed with an innate tumor-tropism. Remarkably, ECFC gold enrichement doesn’t affect cell viability and preserves the endothelial lineage characteristics such as capillary morphogenesis and cell migration. We demonstrate that heavily Au-doped ECFCs are able to efficiently warm up the tumor environment, and kill the cancer cells via hyperthermic heating both in vitro as well as in vivo. Thus, we show an excellent thermotransductive property of gold enriched ECFCs and their capability to kill melanoma cells at moderate NIR light intensities.
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