Oncotarget

Research Papers: Gerotarget (Focus on Aging):

Age, serum 25-hydroxyvitamin D and vitamin D receptor (VDR) expression and function in peripheral blood mononuclear cells

Laura A. Coleman, Margarita Mishina, Mark Thompson, Sarah M. Spencer, Adrian J. Reber, William G. Davis, Po-Yung Cheng, Edward A. Belongia, H. Keipp Talbot, Maria E. Sundaram, Marie R. Griffin, David K. Shay and Suryaprakash Sambhara _

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Oncotarget. 2016; 7:35512-35521. https://doi.org/10.18632/oncotarget.9398

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Abstract

Laura A. Coleman1,4,*, Margarita Mishina2,*, Mark Thompson3, Sarah M. Spencer2, Adrian J. Reber3, William G. Davis3, Po-Yung Cheng3, Edward A. Belongia4, H. Keipp Talbot5, Maria E. Sundaram4,6, Marie R. Griffin5, David K. Shay3 and Suryaprakash Sambhara3

1 Abbott Nutrition, Columbus, OH, USA

2 Battelle, Columbus, OH, USA

3 U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA

4 Marshfield Clinic, Marshfield, WI, USA

5 Vanderbilt University, Nashville, TN, USA

6 University of Minnesota School of Public Health, Minneapolis, MN, USA

* These authors have contributed equally to this work

Correspondence to:

Laura A. Coleman, email:

Keywords: vitamin D, vitamin D receptor, 1α-hydroxylase, mRNA expression, PBMC, Gerotarget

Received: March 06, 2016 Accepted: April 27, 2016 Published: May 17, 2016

Abstract

The relationship between age, vitamin D status, expression and functionality of the vitamin D receptor (VDR), and key genes in the vitamin D pathway in immune cells is unclear. We enrolled adults 50 to 69 years old (20 subjects) and 70+ (20 subjects) and measured: 1) 25(OH)D levels by liquid chromatography/mass spectrometry; and 2) mRNA expression of VDR, 1α-OHase, 1,25D3-MARRS, TREM-1, cathelicidin, RIG-I, and interferon-β by qRT-PCR. Mean serum 25(OH)D was 30 ± 4 ng/mL and was not associated with age. Baseline expression of VDR, 1α-OHase, 1,25D3-MARRS, TREM-1, and RIG-I also did not differ by age; IFN-β expression, however, was higher in the 70+ year old group. 25(OH)D3- and 1,25(OH)2D3-induced VDR, TREM-1 and cathelicidin expression were similar between age groups, as was LPS-induced expression of VDR and of 1α-OHase. Ligand-induced 1,25D3-MARRS expression was higher in subjects ≥ 70 years. Serum 25(OH)D was inversely associated with LPS-stimulated VDR expression and with baseline or vitamin D-induced TREM-1 expression, adjusting for age, self-rated health, and functional status. In healthy adults ≥ 50 years, the expression and functionality of the VDR, 1α-OHase and key vitamin D pathway genes were not consistently associated with age.


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