Polymorphisms in the CHIT1 gene: Associations with colorectal cancer
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Fei-Feng Li1,2,*, Peng Yan3,*, Zhi-Xun Zhao3, Zheng Liu4, Da-Wei Song3, Xing-Wang Zhao3, Xi-Shan Wang2,4, Gui-Yu Wang2,3, Shu-Lin Liu1,2,5
1Genomics Research Center, State-Province Key Laboratory of Biopharmaceutical Engineering, Harbin Medical University, Harbin, China
2Translational Medicine Research and Cooperation Center of Northern China, Heilongjiang Academy of Medical Sciences, Heilongjiang, China
3Department of Colorectal Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China
4Department of Colorectal Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
5Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Canada
*These authors are contributed equally to this work
Shu-Lin Liu, email: firstname.lastname@example.org
Gui-Yu Wang, email: email@example.com
Xi-Shan Wang, email: firstname.lastname@example.org
Keywords: colorectal cancer, CHIT1, C-reaction protein, gene expression, microbe
Received: January 10, 2016 Accepted: April 16, 2016 Published: May 02, 2016
Colorectal cancer (CRC) is one of the most common solid tumors worldwide, often associated with inflammation. The microbes in the human intestine have a key role in inflammations and CRC. Chitotriose renders growth advantage to some bacteria, especially some pathogens, and thus has a role in inflammations. The enzyme chitotriosidase, encoded by the CHIT1 gene of the host, may degrade chitotriose with different efficiencies depending on the alleles. We sequenced the CHIT1 gene for 320 Chinese Han CRC patients and 404 normal controls, and focused on variations rs61745299 and rs35920428 within the CHIT1 gene for their possible roles in CRC. Statistical analyses were conducted using Chi-Square Tests as implemented in SPSS (version 19.0). Multiple sequence alignment was conducted using the Vector NTI, and protein expression levels were analyzed by western blotting. The two variations, rs61745299 and rs35920428 within the CDS region of CHIT1 gene, were associated with the risk of CRC (both with P values < 0.001). Western blotting analysis showed that the variations increased the expression levels of the CHIT1 and C-reaction protein genes in the cancer tissue. We conclude that the two variations of CHIT1, rs61745299 and rs35920428, increase expression of the gene and are associated with CRC in Chinese Han populations.
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