Androgen suppresses testicular cancer cell growth in vitro and in vivo
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Hideo Nakagawa1, Takashi Ueda1, Saya Ito1, Takumi Shiraishi1, Hidefumi Taniguchi1, Naruhiro Kayukawa1, Hiroyuki Nakanishi1, So Ushijima1, Motohiro Kanazawa1, Terukazu Nakamura1, Yoshio Naya1, Fumiya Hongo1, Kazumi Kamoi1, Koji Okihara1, Osamu Ukimura1
1Department of Urology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan
Takashi Ueda, e-mail: email@example.com
Keywords: androgen, androgen receptor, testicular cancer, seminoma, tryptophan hydroxylase 1
Received: December 02, 2015 Accepted: April 11, 2016 Published: April 29, 2016
Silencing of androgen receptor (AR)-meditated androgen signaling is thought to be associated with the development of testicular germ cell tumors (TGCTs). However, the role of the androgen/AR signal in TGCT development has not been investigated. In this study, we show that the androgen/AR signal suppressed the cell growth of seminomas (SEs), a type of TGCT, in vitro and in vivo. Growth of SE cells was suppressed by DHT treatment and reduction of androgen levels by surgical castration promoted cancer cell growth in an in vivo xenograft model. Tryptophan hydroxylase 1 (TPH1), the rate limit enzyme in serotonin synthesis, was one of the genes which expression was reduced in DHT-treated SE cells. TPH1 was highly expressed in SE cancer tissues compared with adjacent normal tissues. Activation of androgen/AR signaling in SE cells reduced the expression of TPH1 in SE cells, followed by the reduction of serotonin secretion in cell culture supernatant. These results suggested that silencing of androgen/AR signaling may cause initiation and progression of SE through increase in TPH1 gene expression level.
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