Oncotarget

Research Papers:

microRNA-802/Rnd3 pathway imposes on carcinogenesis and metastasis of fine particulate matter exposure

Xiaobo Li _, Yang Lv, Na Gao, Hao Sun, Runze Lu, Hongbao Yang, Chengcheng Zhang, Qingtao Meng, Shenshen Wu, Ai-Qun Li, Yankai Xia and Rui Chen

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Oncotarget. 2016; 7:35026-35043. https://doi.org/10.18632/oncotarget.9019

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Abstract

Xiaobo Li1, Yang lv2, Na Gao1, Hao Sun1, Runze Lu1, Hongbao Yang3, Chengcheng Zhang1, Qingtao Meng1, Shenshen Wu1, Ai-Qun Li4, Yankai Xia5,*, Rui Chen1,6,*

1Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China

2Department of Histology and Embryology, Hebei North University, Zhangjiakou, 075000, China

3Center for Drug Safety Evaluation and Research, China Pharmaceutical University, Nanjing, 211198, China

4School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan, 430081, China

5Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing 210009, China

6State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China

*These authors have contributed equally to this work

Correspondence to:

Rui Chen, e-mail: 101011816@seu.edu.cn

Yankai Xia, e-mail: yankaixia@njmu.edu.cn

Keywords: PM2.5, microRNA, lung cancer, carcinogenesis, actin

Received: February 19, 2016     Accepted: April 10, 2016     Published: April 26, 2016

ABSTRACT

Recent studies have linked ambient fine particulate matter (PM2.5) to increased lung cancer mortality and morbidity. However, the underlying mechanism causing the adverse effects of PM2.5 is less clear. In the present study, post-transcriptional profiling was used to explore biological pathways involved in PM2.5-induced pulmonary disorders. The carcinogenesis and metastasis of PM2.5 exposure were evaluated by long-term PM2.5 exposure tests. We observed dysregulation of actin in A549 cells line and dysplasia in the lungs of mice exposed to PM2.5. Both PM2.5-exposed cells and animals showed increased Rnd3 expression levels. Moreover, miR-802 mimics rescued actin disorganization in vitro and alveolitis in vivo. Long-term exposure to PM2.5 promoted carcinogenesis and metastasis of pulmonary cells. Decreased miR-802 expression levels in the serum samples of PM2.5-treated mice and individuals from moderately polluted cities were observed. Increased Rnd3 expression levels in lung cancers tissues have been identified by a genome database TCGA, and have been linked to less overall survival probabilities of lung cancer patients. Our findings suggest that dysregulation of actin cytoskeleton and down-regulation of miR-802 expression might be the underlying mechanism involved in the adverse effects of PM2.5 exposure. In addition, long-term exposure to PM2.5 demonstrated strong associations with malignant pulmonary disorders.


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