Oncotarget

Research Papers:

SOX2 functions as a molecular rheostat to control the growth, tumorigenicity and drug responses of pancreatic ductal adenocarcinoma cells

Erin L. Wuebben, Phillip J. Wilder, Jesse L. Cox, James A. Grunkemeyer, Thomas Caffrey, Michael A. Hollingsworth and Angie Rizzino _

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Oncotarget. 2016; 7:34890-34906. https://doi.org/10.18632/oncotarget.8994

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Abstract

Erin L. Wuebben1, Phillip J. Wilder1, Jesse L. Cox2, James A. Grunkemeyer1, Thomas Caffrey1, Michael A. Hollingsworth1, Angie Rizzino1,3

1Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198-5950, USA

2Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska 68198-3135, USA

3Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska 68198-5870, USA

Correspondence to:

Angie Rizzino, e-mail: arizzino@unmc.edu

Keywords: SOX2, pancreatic cancer, MEK inhibitors, AKT inhibitors, drug resistance

Received: January 27, 2016     Accepted: April 16, 2016     Published: April 26, 2016

ABSTRACT

Pancreatic ductal adenocarcinoma (PDAC) is a highly deadly malignancy. Expression of the stem cell transcription factor SOX2 increases during progression of PDAC. Knockdown of SOX2 in PDAC cell lines decreases growth in vitro; whereas, stable overexpression of SOX2 in one PDAC cell line reportedly increases growth in vitro. Here, we reexamined the role of SOX2 in PDAC cells, because inducible SOX2 overexpression in other tumor cell types inhibits growth. In this study, four PDAC cell lines were engineered for inducible overexpression of SOX2 or inducible knockdown of SOX2. Remarkably, inducible overexpression of SOX2 in PDAC cells inhibits growth in vitro and reduces tumorigenicity. Additionally, inducible knockdown of SOX2 in PDAC cells reduces growth in vitro and in vivo. Thus, growth and tumorigenicity of PDAC cells is highly dependent on the expression of optimal levels of SOX2 – a hallmark of molecular rheostats. We also determined that SOX2 alters the responses of PDAC cells to drugs used in PDAC clinical trials. Increasing SOX2 reduces growth inhibition mediated by MEK and AKT inhibitors; whereas knockdown of SOX2 further reduces growth when PDAC cells are treated with these inhibitors. Thus, targeting SOX2, or its mode of action, could improve the treatment of PDAC.


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