Oncotarget

Research Papers:

The long non-coding RNA PARROT is an upstream regulator of c-Myc and affects proliferation and translation

Dubravka Vučićević, Maja Gehre, Sonam Dhamija, Lennart Friis-Hansen, David Meierhofer, Sascha Sauer and Ulf Andersson Ørom _

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Oncotarget. 2016; 7:33934-33947. https://doi.org/10.18632/oncotarget.8985

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Abstract

Dubravka Vučićević1,5, Maja Gehre1,6, Sonam Dhamija2,7, Lennart Friis-Hansen3, David Meierhofer1, Sascha Sauer4 and Ulf Andersson Ørom1

1 Max Planck Institute for Molecular Genetics, Berlin, Germany

2 Medizinische Hochschule Hannover Institute of Biochemistry, Hannover, Germany

3 Department of Clinical Diochemistry, Næstved Hospital, Næstved, Denmark

4 CU Systems Medicine, Würzburg, Germany

5 Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine, Berlin, Germany

6 EMBL, Heidelberg, Germany

7 RNA Biology and Cancer, German Cancer Research Center (DKFZ), Heidelberg, Germany

Correspondence to:

Ulf Andersson Ørom, email:

Keywords: MYC, long ncRNA

Received: April 13, 2016 Accepted: April 16, 2016 Published: April 25, 2016

Abstract

Long non-coding RNAs are important regulators of gene expression and signaling pathways. The expression of long ncRNAs is dysregulated in cancer and other diseases. The identification and characterization of long ncRNAs is often challenging due to their low expression level and localization to chromatin. Here, we identify a functional long ncRNA, PARROT (Proliferation Associated RNA and Regulator Of Translation) transcribed by RNA polymerase II and expressed at a relatively high level in a number of cell lines. The PARROT long ncRNA is associated with proliferation in both transformed and normal cell lines. We characterize the long ncRNA PARROT as an upstream regulator of c-Myc affecting cellular proliferation and translation using RNA sequencing and mass spectrometry following depletion of the long ncRNA. PARROT is repressed during senescence of human mammary epithelial cells and overexpressed in some cancers, suggesting an important association with proliferation through regulation of c-Myc. With this study, we add to the knowledge of cytoplasmic functional long ncRNAs and extent the long ncRNA-Myc regulatory network in transformed and normal cells.


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