The role of histamine in opening blood-tumor barrier

Zeng Wang; Xin-jun Cai; Jing Qin; Fa-Jun Xie; Na Han; Hong-yang Lu

doi:10.18632/oncotarget.8896

Oncotarget

Oncotarget (a primarily oncology-focused, peer-reviewed, open access journal) aims to maximize research impact through insightful peer-review; eliminate borders between specialties by linking different fields of oncology, cancer research and biomedical sciences; and foster application of basic and clinical science.

Its scope is unique. The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. The term was introduced in the inaugural Editorial, Introducing Oncotarget.

As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. Papers will now be published continuously within yearly volumes in their final and complete form and then quickly released to Pubmed.

Subscribe to receive alerts once a paper has been published by Oncotarget.

Impact Journals, LLC is the publisher of Oncotarget: www.impactjournals.com.

Impact Journals is a member of the Wellcome Trust List of Compliant Publishers.

Impact Journals is a member of the Society for Scholarly Publishing.

On December 23, 2022, Oncotarget server experienced a DDoS attack. As a result, Oncotarget site was inaccessible for a few hours. Oncotarget team swiftly dealt with the situation and took it under control. This malicious action will be reported to the FBI.

Research Papers:

The role of histamine in opening blood-tumor barrier

Zeng Wang _, Xin-jun Cai, Jing Qin, Fa-Jun Xie, Na Han and Hong-yang Lu

PDF | HTML | How to cite

Oncotarget. 2016; 7:31299-31310. https://doi.org/10.18632/oncotarget.8896

Metrics: PDF 1702 views | HTML 2191 views | ?

Abstract

Zeng Wang1,2, Xin-jun Cai3, Jing Qin1,4, Fa-Jun Xie1,4, Na Han1,4, Hong-yang Lu1,4

1Zhejiang Key Laboratory of Diagnosis & Treatment Technology on Thoracic Oncology (Lung and Esophagus), Zhejiang Cancer Hospital, Hangzhou, 310022, P.R. China

2Department of Pharmacy, Zhejiang Cancer Hospital, Hangzhou, 310022, P.R. China

3Department of Pharmacy, Integrated Chinese and Western Medicine Hospital of Zhejiang Province, Hangzhou, 310003, P.R. China

4Department of Thoracic Medical Oncology, Zhejiang Cancer Hospital, Hangzhou, 310022, P.R. China

Correspondence to:

Hong-yang Lu, e-mail: [email protected]

Keywords: histamine, H2 receptor, tight junctions-associated proteins, blood-tumor barrier

Received: January 08, 2016 Accepted: March 31, 2016 Published: April 21, 2016

ABSTRACT

Blood-tumor barrier (BTB) reduce the permeability for drugs into tumor tissues. We found that histamine might serve as an essential regulator of BTB function. Further, we aim to determine the role of H2 receptor expression in BTB permeability, and elucidate the underlying mechanisms thereof. Transmission electron microscopy showed that histamine disrupted the integrity of tight junctions (TJ) and increased the number of pinosomes in the cytoplasm. Horseradish peroxidase (HRP) and trans-endothelial resistance detection (TEER) assays revealed that histamine could open BTB and this action was inhibited by cimetidine. Western blot and immunofluorescence assays showed that histamine decreased the expression of tight junction proteins zonula occluden-1(ZO-1), occludin, and claudin-5. Further, quantitative RT-PCR assay showed that the expression of H2 receptor could represent and predicted histamine-induced BTB permeability. In conclusion, histamine opened BTB by down-regulating the TJ-associated proteins. The levels of H2 receptor expression was correlated with the histamine-induced BTB permeability.

All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 8896

Publication Alerts

Research Papers:

The role of histamine in opening blood-tumor barrier

Abstract