miR-590 promotes cell proliferation and invasion in T-cell acute lymphoblastic leukaemia by inhibiting RB1
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Mei-hua Miao1,*, Xue-qiang Ji1,*, Hao Zhang2,*, Jun Xu1, Hong Zhu1, Xue-jun Shao1
1Department of Clinical Laboratory Diagnosis, Children’s Hospital of Soochow University, Suzhou, China
2Department of Hematology, Affiliated Hospital of Jining Medical University, Jining, China
*These authors have contributed equally to this work
Xue-jun Shao, email: firstname.lastname@example.org
Keywords: miR-590, RB1, T-ALL, cancer
Received: January 28, 2016 Accepted: March 02, 2016 Published: March 28, 2016
MicroRNAs play important roles in the pathogenesis of cancers by inhibiting gene expression at posttranscriptional level. Here, we identified that miR-590 and its predicted target gene RB1 are differentially expressed in T-cell acute lymphoblastic leukaemia (T-ALL). The correlation between miR-590 and RB1 was further confirmed in 395 T-ALL patients. In T-ALL cell lines, miR-590 promoted the cell proliferation by increasing G1/S transition. Moreover, migration and invasion assay showed that miR-590 promotes the migration and invasion of T-ALL cells by increasing E-cadherin and inhibiting MMP-9. Luciferase assays confirmed that miR-590 directly binds to the 3'untranslated region of RB1, and western blotting showed that miR-590 suppresses the expression of RB1 at the protein levels. This study indicated that miR-590 inhibits RB1 and promotes proliferation and invasion of T-ALL cells. Thus, miR-590 may represent a potential therapeutic target for T-ALL intervention.
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