Oncotarget

Research Papers:

miR-590 promotes cell proliferation and invasion in T-cell acute lymphoblastic leukaemia by inhibiting RB1

Mei-hua Miao _, Xue-qiang Ji, Hao Zhang, Jun Xu, Hong Zhu and Xue-jun Shao

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Oncotarget. 2016; 7:39527-39534. https://doi.org/10.18632/oncotarget.8414

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Abstract

Mei-hua Miao1,*, Xue-qiang Ji1,*, Hao Zhang2,*, Jun Xu1, Hong Zhu1, Xue-jun Shao1

1Department of Clinical Laboratory Diagnosis, Children’s Hospital of Soochow University, Suzhou, China

2Department of Hematology, Affiliated Hospital of Jining Medical University, Jining, China

*These authors have contributed equally to this work

Correspondence to:

Xue-jun Shao, email: xuejunshao@hotmail.com

Keywords: miR-590, RB1, T-ALL, cancer

Received: January 28, 2016     Accepted: March 02, 2016     Published: March 28, 2016

ABSTRACT

MicroRNAs play important roles in the pathogenesis of cancers by inhibiting gene expression at posttranscriptional level. Here, we identified that miR-590 and its predicted target gene RB1 are differentially expressed in T-cell acute lymphoblastic leukaemia (T-ALL). The correlation between miR-590 and RB1 was further confirmed in 395 T-ALL patients. In T-ALL cell lines, miR-590 promoted the cell proliferation by increasing G1/S transition. Moreover, migration and invasion assay showed that miR-590 promotes the migration and invasion of T-ALL cells by increasing E-cadherin and inhibiting MMP-9. Luciferase assays confirmed that miR-590 directly binds to the 3'untranslated region of RB1, and western blotting showed that miR-590 suppresses the expression of RB1 at the protein levels. This study indicated that miR-590 inhibits RB1 and promotes proliferation and invasion of T-ALL cells. Thus, miR-590 may represent a potential therapeutic target for T-ALL intervention.


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PII: 8414