Oncotarget

Research Papers:

Phosphorylation of TOPK at Y74, Y272 by Src increases the stability of TOPK and promotes tumorigenesis of colon

Juanjuan Xiao _, Qiuhong Duan, Zhe Wang, Wei Yan, Huimin Sun, Peipei Xue, Xiaoming Fan, Xiaoyu Zeng, Juan Chen, Chen Shao and Feng Zhu

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Oncotarget. 2016; 7:24483-24494. https://doi.org/10.18632/oncotarget.8231

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Abstract

Juanjuan Xiao1,4,*, Qiuhong Duan1,*, Zhe Wang3, Wei Yan3, Huimin Sun2, Peipei Xue1, Xiaoming Fan1, Xiaoyu Zeng1, Juan Chen1, Chen Shao2, Feng Zhu1

1Department of Biochemistry and Molecular Biology, School of Basic Medicine, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, PR China

2Department of Urology, Xijing Hospital, the Fourth Military Medical University, Xi’an, Shaanxi, 710032, PR China

3Department of Pathology, Xijing Hospital, the Fourth Military Medical University, Xi’an, Shaanxi, 710032, PR China

4Laboratory for Translational Oncology Basic Medicine College, Hubei University of Science and Technology, Xianning, Hubei, 437100, PR China

*Juanjuan Xiao and Qiuhong Duan contributed equally to this article and should be considered as co-first authors

Correspondence to:

Juan Chen, e-mail: chenjuanlinda69@163.com

Chen Shao, e-mail: shaochen@fmmu.edu.cn

Feng Zhu, e-mail: fengzhu@hust.edu.cn

Keywords: TOPK, Src, colon cancer, tumorigenesis, stability

Received: January 06, 2016    Accepted: March 04, 2016    Published: March 21, 2016

ABSTRACT

T-LAK cell-originated protein kinase (TOPK), a serine/threonine protein kinase, is highly expressed in a variety of tumors and associated with a poor prognosis of human malignancies. However, the activation mechanism of TOPK is still unrevealed. Herein, first we found that Src directly bound with and phosphorylated TOPK at Y74 and Y272 in vitro. Anti-phospho-TOPK at Y74 was prepared, the endogenous phosphorylation of TOPK at Y74 was detected in colon cancer cells, and the phosphorylation was inhibited in cells expressing low levels of Src. Subsequently, we stably transfected Y74 and Y272 double mutated TOPK (TOPK-FF) into JB6 or SW480 cells, and observed that both the anchorage-independent growth ability and tumorigenesis of TOPK-FF cells were suppressed compared with those of wild type TOPK (TOPK-WT) ex vivo and in vivo. The phosphorylation level of TOPK substrate, Histone H3 at Ser10 also decreased dramatically ex vivo or in vivo. Moreover, we showed that Src could inhibit the ubiquitination of TOPK. Transiently expressed TOPK-WT was more stable than TOPK-FF in pause and chase experiment. Endogenous TOPK was more stable in Src wild type (Src+/+) MEFs than in Src knockout (Src-/-). Taken together, our results indicate that Src is a novel upstream kinase of TOPK. The phosphorylation of TOPK at Y74 and Y272 by Src increases the stability and activity of TOPK, and promotes the tumorigenesis of colon cancer. It may provide opportunities for TOPK based prognosis and targeted therapy for colon cancer patients.


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