Integrated analysis of long non-coding RNAs in human colorectal cancer
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Xiaohua Chen1,2, Binjian Liu2, Rui Yang3, Yong Guo4, Feng Li1, Lin Wang1, Hanyang Hu1
1School of Basic Medical Sciences, Wuhan University, Wuhan, China
2Department of Laboratory Medicine, No.161 Hospital of PLA, Wuhan, China
3Department of General Surgery, No.161 Hospital of PLA, Wuhan, China
4Department of Pathology, No.161 Hospital of PLA, Wuhan, China
Hanyang Hu, e-mail: firstname.lastname@example.org
Keywords: lncRNA, colorectal cancer, expression profiling, epigenetic regulation, poor-prognosis
Received: August 27, 2015 Accepted: February 28, 2016 Published: March 19, 2016
Accumulating evidence highlights the role of long non-coding RNAs (lncRNAs) in tumors. However, the genome-wide expression and roles of lncRNAs in colorectal cancer (CRC) remain unknown. Here, we systematically examined the global gene expressions in primary and synchronous liver metastases CRC tissue, in which thousands of aberrantly expressed lncRNAs were characterized. Co-expression analysis revealed that some lncRNAs correlated to their neighboring mRNAs in expression levels, whereas others formed networks with protein-coding genes in trans. We observed H3K4me3 was enriched at expressed lncRNA transcription start sites (TSSs) and correlated to dysregulated lncRNAs. Furthermore, we identified primary and metastasis tumor linked lncRNA signatures positively correlated with poor-prognosis gene set. Finally, functional experiments demonstrated two candidate lncRNAs were required for proliferation and migration of CRC cells. In summary, we provided a new framework for lncRNA associated clinical prognosis evaluation and target selection of gene therapy in CRC.
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