Oncotarget

Research Papers: Gerotarget (Focus on Aging):

Age-related changes in the transcriptome of antibody-secreting cells

Senthil Kannan, Noor Dawany, Raj Kurupati, Louise C. Showe and Hildegund C.J. Ertl _

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Oncotarget. 2016; 7:13340-13353. https://doi.org/10.18632/oncotarget.7958

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Abstract

Senthil Kannan1,2,*, Noor Dawany2,3,*, Raj Kurupati1, Louise C. Showe2 and Hildegund C.J. Ertl2

1 Gene Therapy and Vaccines Program, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA

2 The Wistar Institute Vaccine Center, Philadelphia, Pennsylvania, USA

3 Department of Biomedical and Health Informatics, The Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA

* These authors have contributed equally to this work

Correspondence to:

Hildegund C.J. Ertl, email:

Keywords: ASCs, B cells, cell metabolism, programmed cell death, oxidative phosphorylation, Gerotarget

Received: November 02, 2015 Accepted: January 28, 2016 Published: March 07, 2016

Abstract

We analyzed age-related defects in B cell populations from young and aged mice. Microarray analysis of bone marrow resident antibody secreting cells (ASCs) showed significant changes upon aging, affecting multiple genes, pathways and functions including those that play a role in immune regulation, humoral immune responses, chromatin structure and assembly, cell metabolism and the endoplasmic reticulum (ER) stress response. Further analysis showed upon aging defects in energy production through glucose catabolism with reduced oxidative phosphorylation. In addition aged B cells had increased levels of reactive oxygen-species (ROS), which was linked to enhanced expression of the co-inhibitor programmed cell death (PD)-1.


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