Oncotarget

Research Papers: Pathology:

Acidic leucine-rich nuclear phosphoprotein-32A (ANP32A) association with lymph node metastasis predicts poor survival in oral squamous cell carcinoma patients

Bharath Kumar Velmurugan, Kun-Tu Yeh, Chien-Hung Lee, Shu-Hui Lin, Mei-Chung Chin, Shang-Lun Chiang, Zhi-Hong Wang, Chun-Hung Hua, Ming-Hsui Tsai, Jan-Gowth Chang and Ying-Chin Ko _

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Oncotarget. 2016; 7:10879-10890. https://doi.org/10.18632/oncotarget.7681

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Abstract

Bharath Kumar Velmurugan1,*, Kun-Tu Yeh2,3,*, Chien-Hung Lee4, Shu-Hui Lin2,3, Mei-Chung Chin2, Shang-Lun Chiang1,5, Zhi-Hong Wang1, Chun-Hung Hua6, Ming-Hsui Tsai6, Jan-Gowth Chang7 and Ying-Chin Ko1,8

1 Environment-Omics-Diseases Research, China Medical University Hospital, Taichung, Taiwan

2 Department of Pathology, Changhua Christian Hospital, Changhua, Taiwan

3 School of Medicine, Chung Shan Medical University, Taichung, Taiwan

4 Department of Public Health, College of Health Science, Kaohsiung Medical University, Kaohsiung, Taiwan

5 Department of Health Risk Management, College of Public Health, China Medical University, Taichung, Taiwan

6 Department of Otorhinolaryngology, China Medical University Hospital, Taichung, Taiwan

7 Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan

8 Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan

* These authors have contributed equally to this work

Correspondence to:

Ying-Chin Ko, email:

Keywords: OSCC, Claudlin-1, ANP32A, Slug, EMT, Pathology Section

Received: November 15, 2015 Accepted: February 16, 2016 Published: February 24, 2016

Abstract

Acidic leucine-rich nuclear phosphoprotein-32A (ANP32A) is a multifunctional protein aberrantly expressed in various types of cancers. However, its expression pattern and clinical significance in oral squamous cell carcinoma (OSCC) remains unclear. In this study, we immunohistochemically investigated the expression pattern of ANP32A in 259 OSCC patients and the results were correlated with clinicopathological factors using Allred, Klein and Immunoreactive scoring (IRS) system. Our data indicated that high expression of ANP32A was significantly associated with N stage and tumor differentiation status in OSCC patients. High ANP32A expression with N2/N3 stage had an increased mortality risk than low ANP32A expressing OSCC patients with N0/N1 stage. Functional studies revealed that knockdown of ANP32A significantly decreased the migration and invasion ability thereby concomitantly increasing E-cadherin and decreasing Slug, Claudin-1 and Vimentin expression in vitro. These results suggest that ANP32A is commonly increased in oral squamous cell carcinoma and ANP32A protein could act as a potential biomarker for prognosis assessment of oral cancer patients with lymph node metastasis.


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