Role of CD44 high /CD133 high  HCT-116 cells in the tumorigenesis of colon cancer

Jin-Yong Zhou; Min Chen; Long Ma; Xiaoxiao Wang; Yu-Gen Chen; Shen-Lin Liu

doi:10.18632/oncotarget.7084

Oncotarget

Oncotarget (a primarily oncology-focused, peer-reviewed, open access journal) aims to maximize research impact through insightful peer-review; eliminate borders between specialties by linking different fields of oncology, cancer research and biomedical sciences; and foster application of basic and clinical science.

Its scope is unique. The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. The term was introduced in the inaugural Editorial, Introducing Oncotarget.

As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. Papers will now be published continuously within yearly volumes in their final and complete form and then quickly released to Pubmed.

Subscribe to receive alerts once a paper has been published by Oncotarget.

Impact Journals, LLC is the publisher of Oncotarget: www.impactjournals.com.

Impact Journals is a member of the Wellcome Trust List of Compliant Publishers.

Impact Journals is a member of the Society for Scholarly Publishing.

On December 23, 2022, Oncotarget server experienced a DDoS attack. As a result, Oncotarget site was inaccessible for a few hours. Oncotarget team swiftly dealt with the situation and took it under control. This malicious action will be reported to the FBI.

Research Papers:

Role of CD44^high/CD133^high HCT-116 cells in the tumorigenesis of colon cancer

Jin-Yong Zhou, Min Chen, Long Ma, Xiaoxiao Wang, Yu-Gen Chen and Shen-Lin Liu _

PDF | HTML | How to cite

Oncotarget. 2016; 7:7657-7666. https://doi.org/10.18632/oncotarget.7084

Metrics: PDF 2628 views | HTML 3651 views | ?

Abstract

Jin-Yong Zhou1,*, Min Chen2,*, Long Ma3,*, Xiaoxiao Wang4, Yu-Gen Chen5,*, Shen-Lin Liu6

1Central Laboratory, The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, China

2Department of Internal Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, China

3Department of Urology, The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, China

4Department of Medical Science Research, The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, China

5Department of Colorectal Surgery, The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, China

6Department of Oncology, The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, China

*These authors contributed equally to this work

Correspondence to:

Shen-Lin Liu, e-mail: [email protected]

Keywords: CD133, CD44, cancer stem cells (CSCs), colorectal cancer, biomarkers

Received: September 24, 2015 Accepted: January 13, 2016 Published: January 30, 2016

ABSTRACT

This study aimed to explore cell surface biomarkers related to cancer stem cells (CSCs) and their role in the tumorigenesis of colon cancer. Various colon cancer cell lines were screened for CD133 and CD44 expression. CD44^high/CD133^high and CD44^low/CD133^low cells were separately isolated by Fluorescence-Activated Cell Sorting (FACS). The cell proliferation, colony formation, cell cycle characteristics, and tumorigenic properties in CD44^high/CD133^high and CD44^low/CD133^low cells were investigated through in vitro experiments and in vivo tumor xenograft models. The expression profiles of stem cell-related genes were examined by RT-PCR. With HCT-116 cells, flow cytometry analysis revealed that CD44^high/CD133^high cells had higher proliferation potency than CD44^low/CD133^low cells. Compared to CD44^low/CD133^low cells, CD44^high/CD133^high cells had more stem cell-related genes, and displayed increased tumorigenic ability. In summary, CD44^high/CD133^high cells isolated from HCT-116 cells harbor CSC properties that may be related to the tumor growth of colon cancer. These results suggest that CD44 and CD133 could be strong markers of colorectal cancer stem cells.

All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 7084

Publication Alerts

Research Papers:

Role of CD44high/CD133high HCT-116 cells in the tumorigenesis of colon cancer

Abstract

Role of CD44^high/CD133^high HCT-116 cells in the tumorigenesis of colon cancer