Oncotarget

Research Papers:

Lentivirus-mediated PLCγ1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma

Bingchang Zhang, Fen Wang, Lianzhi Dai, Heguo Cai, Yanyan Zhan, Song Gang, Tianhui Hu, Chun Xia _ and Bing Zhang

PDF  |  HTML  |  Order a Reprint

Oncotarget. 2016; 7:8043-8054. https://doi.org/10.18632/oncotarget.6976

Metrics: PDF 1034 views  |   HTML 891 views  |   ?  


Abstract

Bingchang Zhang1, Fen Wang1, Lianzhi Dai1, Heguo Cai2, Yanyan Zhan1, Song Gang1, Tianhui Hu1, Chun Xia2, Bing Zhang1

1School of Medicine, Xiamen University, Fujian, 361102, China

2Zhongshan Hospital, Xiamen University, Fujian, 361004, China

Correspondence to:

Chun Xia, e-mail: chunxia@xmu.edu.cn

Bing Zhang, e-mail: cristal66@xmu.edu.cn

Keywords: PLCγ1, ShRNA, tumor growth, tumor metastasis, human gastric adenocarcinoma cells

Received: September 09, 2015     Accepted: January 01, 2016     Published: January 22, 2016

ABSTRACT

Targeted molecular therapy has gradually been a potential solution in cancer therapy. Other authors’ and our previous studies have demonstrated that phosphoinositide-specific phospholipase γ (PLCγ) is involved in regulating tumor growth and metastasis. However, the molecular mechanism underlying PLCγ-dependent tumor growth and metastasis of gastric adenocarcinoma and whether PLCγ may be a potential target for tumor therapy in human gastric adenocarcinoma are not yet well determined. Here, we investigated the role of PLCγ inhibition in tumor growth and metastasis of human gastric adenocarcinoma using BGC-823 cell line and a nude mouse tumor xenograft model. The results manifested that the depletion of PLCγ1 by the transduction with lentivirus-mediated PLCγ1 gene short-hairpin RNA (shRNA) vector led to the decrease of tumor growth and metastasis of human gastric adenocarcinoma in vitro and in vivo. Furthermore, the Akt/Bad, Akt/S6, and ERK/Bad signal axes were involved in PLCγ1-mediated tumor growth and metastasis of human gastric adenocarcinoma. Therefore, the abrogation of PLCγ1 signaling by shRNA could efficaciously suppress human gastric adenocarcinoma tumor growth and metastasis, with important implication for validating PLCγ1 as a potential target for human gastric adenocarcinoma.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 6976