Oncotarget

Research Papers:

Dlx-2 and glutaminase upregulate epithelial-mesenchymal transition and glycolytic switch

Su Yeon Lee _, Hyun Min Jeon, Min Kyung Ju, Eui Kyong Jeong, Cho Hee Kim, Hye Gyeong Park, Song Iy Han and Ho Sung Kang

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Oncotarget. 2016; 7:7925-7939. https://doi.org/10.18632/oncotarget.6879

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Abstract

Su Yeon Lee1, Hyun Min Jeon1, Min Kyung Ju1, Eui Kyong Jeong1, Cho Hee Kim1,*, Hye Gyeong Park2, Song Iy Han3, Ho Sung Kang1

1Department of Molecular Biology, College of Natural Sciences, Pusan 609-735, Korea

2Nanobiotechnology Center, Pusan National University, Pusan 609-735, Korea

3The Division of Natural Medical Sciences, College of Health Science, Chosun University, Gwangju 501-759, Korea

*Present address: DNA Identification Center, National Forensic Service, Seoul 158-707, Korea

Correspondence to:

Ho Sung Kang, e-mail: hspkang@pusan.ac.kr

Keywords: Dlx-2, GLS1, Snail, epithelial-mesenchymal transition, glycolytic switch

Received: May 23, 2015     Accepted: January 02, 2016     Published: January 11, 2016

ABSTRACT

Most cancer cells depend on enhanced glucose and glutamine (Gln) metabolism for growth and survival. Oncogenic metabolism provides biosynthetic precursors for nucleotides, lipids, and amino acids; however, its specific roles in tumor progression are largely unknown. We previously showed that distal-less homeobox-2 (Dlx-2), a homeodomain transcription factor involved in embryonic and tumor development, induces glycolytic switch and epithelial-mesenchymal transition (EMT) by inducing Snail expression. Here we show that Dlx-2 also induces the expression of the crucial Gln metabolism enzyme glutaminase (GLS1), which converts Gln to glutamate. TGF-β and Wnt induced GLS1 expression in a Dlx-2-dependent manner. GLS1 shRNA (shGLS1) suppressed in vivo tumor metastasis and growth. Inhibition of Gln metabolism by shGLS1, Gln deprivation, and Gln metabolism inhibitors (DON, 968 and BPTES) prevented Dlx-2-, TGF-β-, Wnt-, and Snail-induced EMT and glycolytic switch. Finally, shDlx-2 and Gln metabolism inhibition decreased Snail mRNA levels through p53-dependent upregulation of Snail-targeting microRNAs. These results demonstrate that the Dlx-2/GLS1/Gln metabolism axis is an important regulator of TGF-β/Wnt-induced, Snail-dependent EMT, metastasis, and glycolytic switch.


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