Oncotarget

Research Papers: Immunology:

GITRL modulates the activities of p38 MAPK and STAT3 to promote Th17 cell differentiation in autoimmune arthritis

Xinyi Tang, Jie Tian, Jie Ma, Jiemin Wang, Chen Qi, Ke Rui, Yungang Wang, Huaxi Xu, Liwei Lu and Shengjun Wang _

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Oncotarget. 2016; 7:8590-8600. https://doi.org/10.18632/oncotarget.6535

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Abstract

Xinyi Tang1,*, Jie Tian2,*, Jie Ma2, Jiemin Wang2, Chen Qi2, Ke Rui2, Yungang Wang2, Huaxi Xu2, Liwei Lu3 and Shengjun Wang1,2

1 Department of Laboratory Medicine, The Affiliated People’s Hospital, Jiangsu University, Zhenjiang, China

2 Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University, Zhenjiang, China

3 Department of Pathology, The University of Hong Kong, Hong Kong, China

* These authors have contributed equally to this work

Correspondence to:

Shengjun Wang, email:

Keywords: GITRL, p38 MAPK, STAT3, Th17 cells, autoimmune arthritis, Immunology and Microbiology Section, Immune response, Immunity

Received: September 05, 2015 Accepted: November 26, 2015 Published: December 09, 2015

Abstract

The glucocorticoid-induced TNFR family-related protein (GITR) and its ligand play a critical role in the pathogenesis of autoimmune arthritis by enhancing the Th17 cell response, but their molecular mechanisms remain largely unclear. This study aims to define the role of p38 mitogen-activated protein kinases (MAPK) and signal transducer and activator of transcription 3 (STAT3) signaling in GITRL-induced Th17 cells in autoimmune arthritis. We found that the p38 phosphorylation was enhanced by GITRL in activated CD4+T cells, and the p38 inhibitor restrained the GITRL-induced Th17 cell expansion in a dose-dependent manner. Moreover, there was decreased STAT3 activity on Tyr705 and Ser727 with the p38 inhibitor in vitro. Notably, the p38 inhibitor could prevent GITRL-treated arthritis progression and markedly decrease the Th17 cell percentages. The phosphorylation of the Tyr705 site was significantly lower in the GITRL-treated CIA mice administrated with the p38 inhibitor. A significantly higher phosphorylation of p38 was detected in RA patients and had a positive relationship with the serum level of anti-cyclic citrullinated peptide (anti-CCP) antibody. Our findings have indicated that GITRL could promote Th17 cell differentiation by p38 MAPK and STAT3 signaling in autoimmune arthritis.


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