Transcription factor LSF (TFCP2) inhibits melanoma growth
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Yuji Goto1,2, Ichiro Yajima1,3, Mayuko Kumasaka1,3, Nobutaka Ohgami1,3, Asami Tanaka1, Toyonori Tsuzuki4, Yuji Inoue5, Satoshi Fukushima5, Hironobu Ihn5, Mikiko Kyoya6, Hiroyuki Ohashi6, Tamihiro Kawakami6, Dorothy C. Bennett7 and Masashi Kato1,3
1 Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Matsumoto-cho, Kasugai-shi, Aichi, Japan
2 Department of Biology, Faculty of Science, Toho University, Miyama, Funabashi, Japan
3 Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Tsurumai-cho, Showa-ku, Nagoya, Aichi, Japan
4 Department of Pathology, Nagoya Daini Red Cross Hospital, Nagoya, Aichi, Japan
5 Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan
6 Department of Dermatology, St. Marianna University School of Medicine, Sugao, Miyamae-ku, Kawasaki, Kanagawa, Japan
7 Molecular Cell Sciences Research Centre, St George’s, University of London, London, UK
Masashi Kato, email:
Keywords: melanoma, transcription factor LSF, TFCP2, CDKN1A, cell cycle
Received: May 28, 2015 Accepted: October 09, 2015 Published: October 25, 2015
Late SV40 factor 3 (LSF), a transcription factor, contributes to human hepatocellular carcinoma (HCC). However, decreased expression level of LSF in skin melanoma compared to that in benign melanocytic tumors and nevi in mice and humans was found in this study. Anchorage-dependent and -independent growth of melanoma cells was suppressed by LSF overexpression through an increased percentage of G1 phase cells and an increased p21CIP1 expression level in vitro and in vivo. Anchorage-dependent growth in LSF-overexpressed melanoma cells was promoted by depletion of LSF in the LSF-overexpressed cells. Integrated results of our EMSA and chromatin immunoprecipitation assays showed binding of LSF within a 150-bp upstream region of the transcription start site of p21CIP1 in melanoma cells. Taken together, our results suggest potential roles of LSF as a growth regulator through control of the transcription of p21CIP1 in melanocytes and melanoma cells as well as a biomarker for nevus.
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