Oncotarget

Priority Research Papers:

Chromosome transplantation as a novel approach for correcting complex genomic disorders

Marianna Paulis, Alessandra Castelli, Lucia Susani, Michela Lizier, Irina Lagutina, Maria Luisa Focarelli, Camilla Recordati, Paolo Uva, Francesca Faggioli, Tui Neri, Eugenio Scanziani, Cesare Galli, Franco Lucchini, Anna Villa and Paolo Vezzoni _

PDF  |  HTML  |  Supplementary Files  |  How to cite  |  Order a Reprint

Oncotarget. 2015; 6:35218-35230. https://doi.org/10.18632/oncotarget.6143

Metrics: PDF 1053 views  |   HTML 1298 views  |   ?  


Abstract

Marianna Paulis1,2, Alessandra Castelli1,2, Lucia Susani1,2, Michela Lizier2,3, Irina Lagutina4, Maria Luisa Focarelli1,2, Camilla Recordati5, Paolo Uva6, Francesca Faggioli1,2, Tui Neri1,2, Eugenio Scanziani5,7, Cesare Galli4,8, Franco Lucchini3, Anna Villa1,2,* and Paolo Vezzoni1,2,*

1 Milan Unit, Istituto di Ricerca Genetica e Biomedica, CNR, Milan, Italy

2 Humanitas Clinical and Research Center, Rozzano, Milan, Italy

3 Centro Ricerche Biotecnologiche, Università Cattolica del Sacro Cuore, Cremona, Italy

4 Avantea, Cremona, Italy

5 Mouse and Animal Pathology Laboratory, Fondazione Filarete, Milan, Italy

6 CRS4 Bioinformatics Laboratory, Parco Scientifico e Tecnologico POLARIS, Pula, Cagliari, Italy

7 Department of Veterinary Sciences and Public Health, University of Milan, Milan, Italy

8 Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia, Bologna, Italy

* These authors jointly supervised this work

Correspondence to:

Paolo Vezzoni, email:

Marianna Paulis, email:

Keywords: embryonic stem cells, chromosome transplantation, microcell fusion, genomic disorders, cell therapy

Received: September 28, 2015 Accepted: October 01, 2015 Published: October 17, 2015

Abstract

Genomic disorders resulting from large rearrangements of the genome remain an important unsolved issue in gene therapy. Chromosome transplantation, defined as the perfect replacement of an endogenous chromosome with a homologous one, has the potential of curing this kind of disorders. Here we report the first successful case of chromosome transplantation by replacement of an endogenous X chromosome carrying a mutation in the Hprt genewith a normal one in mouse embryonic stem cells (ESCs), correcting the genetic defect. The defect was also corrected by replacing the Y chromosome with an X chromosome. Chromosome transplanted clones maintained in vitro and in vivo features of stemness and contributed to chimera formation. Genome integrity was confirmed by cytogenetic and molecular genome analysis. The approach here proposed, with some modifications, might be used to cure various disorders due to other X chromosome aberrations in induced pluripotent stem (iPS) cells derived from affected patients.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 6143