Silencing P2X7 receptor downregulates the expression of TCP-1 involved in lymphoma lymphatic metastasis

Xudong Jiang; Wenjuan Mao; Ziyi Yang; Jia Zeng; Yi Zhang; Yang Song; Ying Kong; Shuangyi Ren; Yunfei Zuo

doi:10.18632/oncotarget.5870

Oncotarget

Oncotarget (a primarily oncology-focused, peer-reviewed, open access journal) aims to maximize research impact through insightful peer-review; eliminate borders between specialties by linking different fields of oncology, cancer research and biomedical sciences; and foster application of basic and clinical science.

Its scope is unique. The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. The term was introduced in the inaugural Editorial, Introducing Oncotarget.

As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. Papers will now be published continuously within yearly volumes in their final and complete form and then quickly released to Pubmed.

Subscribe to receive alerts once a paper has been published by Oncotarget.

Impact Journals, LLC is the publisher of Oncotarget: www.impactjournals.com.

Impact Journals is a member of the Wellcome Trust List of Compliant Publishers.

Impact Journals is a member of the Society for Scholarly Publishing.

On December 23, 2022, Oncotarget server experienced a DDoS attack. As a result, Oncotarget site was inaccessible for a few hours. Oncotarget team swiftly dealt with the situation and took it under control. This malicious action will be reported to the FBI.

Research Papers:

Silencing P2X7 receptor downregulates the expression of TCP-1 involved in lymphoma lymphatic metastasis

Xudong Jiang, Wenjuan Mao, Ziyi Yang, Jia Zeng, Yi Zhang, Yang Song, Ying Kong, Shuangyi Ren and Yunfei Zuo _

PDF | HTML | Supplementary Files | How to cite

Oncotarget. 2015; 6:42105-42117. https://doi.org/10.18632/oncotarget.5870

Metrics: PDF 1430 views | HTML 1939 views | ?

Abstract

Xudong Jiang1,*, Wenjuan Mao1,*, Ziyi Yang1, Jia Zeng1, Yi Zhang1,2, Yang Song1,2, Ying Kong3, Shuangyi Ren2, Yunfei Zuo1

1Department of Clinical Biochemistry, College of Laboratory Diagnostic Medicine, Dalian Medical University, Dalian 116044, China

2Department of Surgery, the Second Affiliated Hospital of Dalian Medical University, Dalian 116023, China

3Department of Biochemistry, College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, China

*These authors have equally contributed to this work

Correspondence to:

Yunfei Zuo, e-mail: [email protected]

Keywords: P2X₇R, TCP-1, lymphoma, proteomics, lymphatic-metastasis

Received: April 18, 2015 Accepted: October 27, 2015 Published: November 06, 2015

ABSTRACT

P2X₇R is an ATP-gated cation channel that participates in cell proliferation and apoptosis. TCP-1 assists with the protein folding. According to our previous research, the P2X₇R has a potential role in P388D1 lymphoid neoplasm cells dissemination to peripheral lymph nodes. In order to make a further exploration about the probable mechanism, the lymph nodes which metastasized by P2X₇R-silenced P388D1 cells or non-silenced cells were analyzed by 2DE and a MALDI-TOF-based proteomics approach. In the 64 proteins which were differentially expressed between two groups, TCP-1 was found to be significantly decreased in P2X₇R shRNA group compared to controls. This correlation was also found in subsequent experiments in vivo and in vitro. The positive correlation between P2X₇R and TCP-1 was also proved in both lymphoma and benign lymphadenopathy tissues from patients. It indicates that TCP-1 may be a crucial downstream molecular of P2X₇R and plays a novel role in lymphoid neoplasm metastasis.

All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 5870

Publication Alerts

Research Papers:

Silencing P2X7 receptor downregulates the expression of TCP-1 involved in lymphoma lymphatic metastasis

Abstract