Oncotarget

Research Papers:

A microfluidic approach towards hybridoma generation for cancer immunotherapy

Yen-Ta Lu _, Gaurav Prashant Pendharkar, Chung-Huan Lu, Chia-Ming Chang and Cheng-Hsien Liu

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Oncotarget. 2015; 6:38764-38776. https://doi.org/10.18632/oncotarget.5550

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Abstract

Yen-Ta Lu1,2,*, Gaurav Prashant Pendharkar3,*, Chung-Huan Lu3, Chia-Ming Chang4, Cheng-Hsien Liu3

1Division of Chest Medicine, Department of Internal Medicine, Mackay Memorial Hospital, Taipei, Taiwan, R.O.C

2Department of Medicine, Mackay Medical College, New Taipei City, Taiwan, R.O.C

3Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan, R.O.C

4Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan

*These authors have contributed equally to this work

Correspondence to:

Cheng-Hsien Liu, e-mail: liuch@pme.nthu.edu.tw

Keywords: immunotherapy, cell electrofusion, hydrodynamic trapping, dielectrophoresis, LabChip

Received: July 23, 2015     Accepted: September 25, 2015     Published: October 07, 2015

ABSTRACT

Dendritic cells/tumor fusions have shown to elicit anti-cancer immunity in different cancer types. However, the application of these vaccines for human cancer immunotherapy are limited by the instable quality and insufficient quanity of fusion cells. We present a cell electrofusion chip fabricated using soft lithography technique, which combines the rapid and precise cell pairing microstructures and the high yield electrofusion micro-electrodes to improve the cell fusion. The design uses hydrodynamic trapping in combination with positive dielectrophoretic force (pDEP) to achieve cell fusion. The chip consists of total 960 pairs of trapping channels, which are capable of pairing and fusing both homogeneous and heterogeneous types of cells. The fused cells can be easily taken out of the chip that makes this device a distinguishable from other designs. We observe pairing efficiency of 68% with fusion efficiency of 64%.


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