Oncotarget

Research Papers:

Genetic variations in the TERT and CLPTM1L gene region and gastrointestinal stromal tumors risk

Rui Zhang, Jian Zhao, Jian Xu, Fang Liu, Yongqing Xu, Xianmin Bu, Chaoliu Dai and Chun Song _

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Oncotarget. 2015; 6:31360-31367. https://doi.org/10.18632/oncotarget.5153

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Abstract

Rui Zhang1, Jian Zhao1, Jian Xu1, Fang Liu1, Yongqing Xu2, Xianmin Bu2, Chaoliu Dai2, Chun Song1

1Department of Colorectal Surgery, Liaoning Cancer Hospital & Institute, Shenyang 110042, Liaoning Province, P.R. China

2Department of Hepatobiliary and Splenic Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning Province, P.R. China

Correspondence to:

Chun Song, e-mail: songchunshenyang@163.com

Keywords: gastrointestinal stromal tumors, GIST, TERT, CLPTM1L, polymorphism

Received: March 10, 2015     Accepted: August 27, 2015     Published: September 08, 2015

ABSTRACT

Recent studies have suggested polymorphisms in the TERT and CLPTM1L region are associated with carcinogenesis of many distinct cancer types, including gastrointestinal cancers. However, the contribution of polymorphisms in the TERT and CLPTM1L gene region to gastrointestinal stromal tumors (GISTs) risk is still unknown. We tested the six tagSNPs on TERT and CLPTM1L region with GIST risk, using a population-based, two-stage, case-control study in 2,000 subjects. Functional validation was conducted to validate our findings of TERT rs2736098 and explore its influence on relative telomere length (RTL) in GIST cells. It showed that variant rs2736098 was significantly associated with increased risk of GIST (per allele OR = 1.29, 95% CI: 1.14–1.47, P = 7.03 × 10−5). The difference remain significant after Bonferroni correction (P = 7.03 × 10−5 * 6 = 4.2 × 10−4). Real-time PCR showed carriers of genotype CC have the longest RTL, following by carriers of genotype CT, while carriers of genotype TT have the shortest RTL in GIST tissues (P < 0.001). Our data provide evidence to implicate TERT rs2736098 polymorphism as a novel susceptibility factor for GIST risk.


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