Specificity protein (Sp) transcription factors and metformin regulate expression of the long non-coding RNA HULC

Shruti U. Gandhy; Parisa Imanirad; Un-Ho Jin; Vijayalekshmi Nair; Eric Hedrick; Yating Cheng; Christopher J. Corton; KyoungHyun Kim; Stephen Safe

doi:10.18632/oncotarget.4560

Oncotarget

Oncotarget (a primarily oncology-focused, peer-reviewed, open access journal) aims to maximize research impact through insightful peer-review; eliminate borders between specialties by linking different fields of oncology, cancer research and biomedical sciences; and foster application of basic and clinical science.

Its scope is unique. The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. The term was introduced in the inaugural Editorial, Introducing Oncotarget.

As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. Papers will now be published continuously within yearly volumes in their final and complete form and then quickly released to Pubmed.

Subscribe to receive alerts once a paper has been published by Oncotarget.

Impact Journals, LLC is the publisher of Oncotarget: www.impactjournals.com.

Impact Journals is a member of the Wellcome Trust List of Compliant Publishers.

Impact Journals is a member of the Society for Scholarly Publishing.

On December 23, 2022, Oncotarget server experienced a DDoS attack. As a result, Oncotarget site was inaccessible for a few hours. Oncotarget team swiftly dealt with the situation and took it under control. This malicious action will be reported to the FBI.

Research Papers:

Specificity protein (Sp) transcription factors and metformin regulate expression of the long non-coding RNA HULC

Shruti U. Gandhy, Parisa Imanirad, Un-Ho Jin, Vijayalekshmi Nair, Eric Hedrick, Yating Cheng, Christopher J. Corton, KyoungHyun Kim and Stephen Safe _

PDF | HTML | Supplementary Files | How to cite

Oncotarget. 2015; 6:26359-26372. https://doi.org/10.18632/oncotarget.4560

Metrics: PDF 2189 views | HTML 2268 views | ?

Abstract

Shruti U. Gandhy1,*, Parisa Imanirad2,*, Un-Ho Jin1,*, Vijayalekshmi Nair2,*, Eric Hedrick2,*, Yating Cheng2, J. Christopher Corton3, KyoungHyun Kim4, Stephen Safe1,2

1Institute of Biosciences and Technology, Texas A&M Health Sciences Center, Houston, TX, USA

2Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX, USA

3Integrated Systems Toxicology Division, US-EPA, MD B143-06, Research Triangle Park, NC, USA

4Department of Environmental Health, University of Cincinnati, Cincinnati, OH, USA

*These authors have contributed equally to this work

Correspondence to:

Stephen Safe, e-mail: [email protected]

Keywords: HCC, Sp proteins, HULC, IncRNAs

Received: May 19, 2015 Accepted: June 23, 2015 Published: July 06, 2015

ABSTRACT

Specificity protein 1 (Sp1) transcription factor (TF) regulates expression of long non-coding RNAs (lncRNAs) in hepatocellular carcinoma (HCC) cells. RNA interference (RNAi) studies showed that among several lncRNAs expressed in HepG2, SNU-449 and SK-Hep-1 cells, highly upregulated in liver cancer (HULC) was regulated not only by Sp1 but also Sp3 and Sp4 in the three cell lines. Knockdown of Sp transcription factors and HULC by RNAi showed that they play important roles in HCC cell proliferation, survival and migration. The relative contribution of Sp1, Sp3, Sp4 and HULC on these responses in HepG2, SNU-449 and SK-Hep-1 cells were cell context- and response-dependent. In the poorly differentiated SK-Hep-1 cells, knockdown of Sp1 or HULC resulted in genomic and morphological changes, indicating that Sp1 and Sp1-regulated HULC are important for maintaining the mesenchymal phenotype in this cell line. Genomic analysis showed an inverse correlation between expression of genes after knockdown of HULC and expression of those genes in liver tumors from patients. The antidiabetic drug metformin down-regulates Sp proteins in pancreatic cancer, and similar results including decreased HULC expression were observed in HepG2, SNU-449 and SK-Hep-1 cells treated with metformin, indicating that metformin and other antineoplastic agents that target Sp proteins may have clinical applications for HCC chemotherapy.

All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 4560

Publication Alerts

Research Papers:

Specificity protein (Sp) transcription factors and metformin regulate expression of the long non-coding RNA HULC

Abstract