Oncotarget

Research Papers: Gerotarget (Focus on Aging):

Differential modulation of AMPK/PPARα/UCP2 axis in relation to hypertension and aging in the brain, kidneys and heart of two closely related spontaneously hypertensive rat strains

Speranza Rubattu _, Franca Bianchi, Carla Letizia Busceti, Maria Cotugno, Rosita Stanzione, Simona Marchitti, Sara Di Castro, Michele Madonna, Ferdinando Nicoletti and Massimo Volpe

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Oncotarget. 2015; 6:18800-18818. https://doi.org/10.18632/oncotarget.4033

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Abstract

Speranza Rubattu1,2, Franca Bianchi2, Carla Letizia Busceti2, Maria Cotugno2, Rosita Stanzione2, Simona Marchitti2, Sara Di Castro2, Michele Madonna2, Ferdinando Nicoletti2,3, Massimo Volpe1,2

1Department of Clinical and Molecular Medicine, School of Medicine and Psychology, Sapienza University of Rome, Ospedale S. Andrea, Rome

2Istituto di Ricovero e Cura a Carattere Scientifico (I.R.C.C.S.) Neuromed, Località Camerelle, Pozzilli, Italy

3Department of Physiology and Pharmacology, Sapienza University of Rome, Rome, Italy

Correspondence to:

Speranza Rubattu, e-mail: rubattu.speranza@neuromed.it

Keywords: uncoupling protein 2, SHRSP, hypertension, target organ damage, aging

Received: May 06, 2015     Accepted: May 09, 2015     Published: May 22, 2015

ABSTRACT

Objectives: We examined expression protein of AMPK/SIRT1/PGC1α/PhoxO3a/PPARα/UCP2 pathway in brain, kidneys and heart of stroke-prone spontaneously hypertensive rat (SHRSP) vs stroke-resistant SHR (SHRSR) at different weeks of age, up to one year, in order to test the hypothesis that abnormalities within this pathway could associate with higher susceptibility of SHRSP to develop hypertension-related vascular damage.

Background: SHRSP develops severe hypertension and related target organ damage. Marked reduction of uncoupling protein 2 (UCP2) expression upon high salt-low potassium diet associates with increased renal injury in SHRSP. UCP2 may represent a key mitochondrial protein involved in cardiovascular damage.

Results: At 2 months of age a significant down-regulation of UCP2 expression at both mRNA and protein levels was found, along with reduced protein expression of all components of UCP2 regulatory pathway, in tissues of SHRSP but not of SHRSR, that progressed with hypertension development and aging. A significant increase of both oxidative stress and inflammation was detected in tissues of SHRSP as a function of age. SBP levels were significantly higher in SHRSP than SHRSR at 3 months of age and thereafter. At one year of age, higher degree of renal damage, with proteinuria and severe glomerular and tubulo-interstitial fibrosis, of cerebral damage, with significant vessel extravasation and stroke occurrence, and of myocardial damage was detected in SHRSP than SHRSR.

Conclusions: The early significant reduced expression of the antioxidant AMPK/PPARα/UCP2 pathway that progressed throughout lifetime may contribute to explain higher predisposition of SHRSP to oxidative stress dependent target organ damage in the context of severe hypertension.


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