Anchoring of both PKA-RIIα and 14-3-3θ regulates retinoic acid induced 16 mediated phosphorylation of heat shock protein 70

Cui-Ling Ding; Gang Xu; Hai-Lin Tang; Shi-Ying Zhu; Lan-Juan Zhao; Hao Ren; Ping Zhao; Zhong-Tian Qi; Wen Wang

doi:10.18632/oncotarget.3702

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Research Papers:

Anchoring of both PKA-RIIα and 14-3-3θ regulates retinoic acid induced 16 mediated phosphorylation of heat shock protein 70

Cui-Ling Ding _, Gang Xu, Hai-Lin Tang, Shi-Ying Zhu, Lan-Juan Zhao, Hao Ren, Ping Zhao, Zhong-Tian Qi and Wen Wang

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Oncotarget. 2015; 6:15540-15550. https://doi.org/10.18632/oncotarget.3702

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Abstract

Cui-Ling Ding1,*, Gang Xu1,*, Hai-Lin Tang1, Shi-Ying Zhu1, Lan-Juan Zhao1, Hao Ren1, Ping Zhao1, Zhong-Tian Qi1 and Wen Wang1

1 Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai, China

* These authors have contributed equally to this work

Correspondence to:

Zhong-Tian Qi, email:

Wen Wang, email:

Keywords: RAI16, Fam160B2, AKAP, PKA-RIIα, 14-3-3θ

Received: January 19, 2015 Accepted: March 05, 2015 Published: March 30, 2015

Abstract

Our previous study reported that retinoic acid induced 16 (RAI16) could enhance tumorigenesis in hepatocellular carcinoma (HCC). However, the cellular functions of RAI16 are still unclear. In this study, by immunoprecipitation and tandem (MS/MS) mass spectrometry analysis, we identified that RAI16 interacted with the type II regulatory subunit of PKA (PKA-RIIα), acting as a novel protein kinase A anchoring protein (AKAP). In addition, RAI16 also interacted with heat shock protein 70 (HSP70) and 14-3-3θ. Further studies indicated that RAI16 mediated PKA phosphorylation of HSP70 at serine 486, resulting in anti-apoptosis events. RAI16 was also phosphorylated by the anchored PKA at serine 325, which promoted the recruitment of 14-3-3θ, which, in turn, inhibited RAI16 mediated PKA phosphorylation of HSP70. These findings offer mechanism insight into RAI16 mediated anti-apoptosis signaling in HCC.

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Research Papers:

Anchoring of both PKA-RIIα and 14-3-3θ regulates retinoic acid induced 16 mediated phosphorylation of heat shock protein 70

Abstract