Deficiency of caspase 3 in tumor xenograft impairs therapeutic effect of measles virus Edmoston strain
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Biao Wang1, Xu Yan2, Qingguo Guo1, Yan Li3, Haiyan Zhang4, JiSheng Xie5, Xin Meng1
1Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences of China Medical University Shenyang, P.R. China
2Department of Prosthodontics, School of Stomatology, China Medical University, Shenyang, P.R. China
3Department of Oncology, Tumour Angiogenesis and Microenvironment Laboratory (TAML), First Affiliated Hospital, Liaoning Medical College, Jinzhou, P.R. China
4Department of Geriatrics, The First Affiliated Hospital, China Medical University, Shenyang, P.R. China
5Department of Ecsomatics, Youjiang Medical College for Nationalities, Baise City, P.R. China
Xin Meng, e-mail: email@example.com
Keywords: caspase-3, interferon alpha, oncolytic therapy, apoptosis, measles virus Edmonston strain
Received: January 20, 2015 Accepted: March 07, 2015 Published: March 26, 2015
The oncolytic measles virus Edmonston (MV-Edm) strain shows considerable oncolytic activity against a variety of human tumors. In this study, we report MV-Edm is able to trigger apoptosis pathways in infected tumor cells and elucidate the roles of cellular apoptosis in the whole oncolytic process. We also show that activated caspase 3, a key executioner of apoptosis, plays key roles in the oncolytic virotherapy. Activated caspase 3 can accelerate viral replication in cervical cancer cells and enhance the killing effects of the virus. Deficiency of caspase 3 either in tumor cells or in tumor xenograft significantly desensitized tumor to oncolysis with MV-Edm. In the infected cells, caspase 3 regulates interferon α release, which can inhibit viral replication in neighboring tumor cells. We propose that caspase-3 activation enhances the oncolytic effects of MV-Edm, thus inhibiting tumor growth in mice.
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