Oncotarget

Research Papers:

A standardized autopsy procurement allows for the comprehensive study of DIPG biology

Madhuri Kambhampati, Jennifer P. Perez, Sridevi Yadavilli, Amanda M. Saratsis, Ashley D. Hill, Cheng-Ying Ho, Eshini Panditharatna, Melissa Markel, Roger J. Packer, Javad Nazarian _

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Oncotarget. 2015; 6:12740-12747. https://doi.org/10.18632/oncotarget.3374

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Abstract

Madhuri Kambhampati1, Jennifer P. Perez1, Sridevi Yadavilli1, Amanda M. Saratsis1,2,3, Ashley D. Hill4, Cheng-Ying Ho4, Eshini Panditharatna1,7, Melissa Markel5, Roger J. Packer6 and Javad Nazarian1,8

1 Research Center for Genetic Medicine, Children’s National Health System, Washington, DC, USA

2 Division of Pediatric Neurosurgery, Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, IL, USA

3 Department of Neurological Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL, USA

4 Division of Pathology, Children’s National Health System, Washington, DC, USA

5 Department of Neuro Oncology, Riley hospital for Children, Indiana University Health, Indianapolis, IN USA

6 Brain Tumor Institute, Center for Neuroscience and Behavioral Medicine, Children’s National Health System, Washington, DC, USA

7 Institute for Biomedical Sciences, George Washington University, Washington, DC, USA

8 Department of Integrative Systems Biology, George Washington University School of Medicine and Health Sciences, Washington, DC, USA

Correspondence to:

Javad Nazarian, email:

Keywords: Diffuse Intrinsic Pontine Glioma (DIPG), Brainstem Glioma, Autopsy, Histone 3, Orthotopic Injection

Received: December 09, 2014 Accepted: January 15, 2015 Published: January 24, 2015

Abstract

Diffuse intrinsic pontine glioma (DIPG) is one of the least understood and most deadly childhood cancers. Historically, there has been a paucity of DIPG specimens for molecular analysis. However, due to the generous participation of DIPG families in programs for postmortem specimen donation, there has been a recent surge in molecular analysis of newly available tumor specimens. Collaborative efforts to share data and tumor specimens have resulted in rapid discoveries in other pediatric brain tumors, such as medulloblastoma, and therefore have the potential to shed light on the biology of DIPG. Given the generous gift of postmortem tissue donation from DIPG patients, there is a need for standardized postmortem specimen accrual to facilitate rapid and effective multi-institutional molecular studies.

We developed and implemented an autopsy protocol for rapid procurement, documenting and storing these specimens. Sixteen autopsies were performed throughout the United States and Canada and processed using a standard protocol and inventory method, including specimen imaging, fixation, snap freezing, orthotopic injection, or preservation. This allowed for comparative clinical and biological studies of rare postmortem DIPG tissue specimens, generation of in vivo and in vitro models of DIPG, and detailed records to facilitate collaborative analysis.


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