Oncotarget

Research Papers:

DACH1 is a novel predictive and prognostic biomarker in hepatocellular carcinoma as a negative regulator of Wnt/β-catenin signaling

Yu Liu _, Rong Zhou, Xun Yuan, Na Han, Si Zhou, Hanxiao Xu, Mingzhou Guo, Shiying Yu, Cuntai Zhang, Tiejun Yin and Kongming Wu

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Oncotarget. 2015; 6:8621-8634. https://doi.org/10.18632/oncotarget.3281

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Abstract

Yu Liu1, Rong Zhou1, Xun Yuan2, Na Han2, Si Zhou1, Hanxiao Xu2, Mingzhou Guo3, Shiying Yu2, Cuntai Zhang1, Tiejun Yin1, Kongming Wu2

1Department of Geriatrics, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. China

2Department of Oncology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. China

3Department of Gastroenterology and Hepatology, Chinese PLA General Hospital, Beijing, P.R. China

Correspondence to:

Tiejun Yin, e-mail: tjyin@tjh.tjmu.edu.cn

Kongming Wu, e-mail: kmwu@tjh.tjmu.edu.cn

Keywords: DACH1, hepatocellular carcinoma, Wnt/β-catenin signaling, prognostic factor, TMA

Received: December 09, 2014     Accepted: February 05, 2015     Published: April 02, 2015

ABSTRACT

The cell fate determination factor Dachshund (DACH1) functions as a novel suppressor in the progression of various neoplasms. Previous study has suggested that hypermethylation of promoter region was responsible for the reduction of DACH1 expression in hepatocellular carcinoma (HCC), and associated with the progression of HCC, but the clinical significance and the exact molecular mechanisms of DACH1 in the progression of HCC remain unclear. In this study, we employed public microarray data analysis and tissue microarrays (TMAs) technologies and showed that DACH1 expression was reduced in HCC even at early stage and associated with the tumor progression. Notably, Kaplan-Meier analysis further indicated DACH1 could be an independent prognostic factor for the overall survival of HCC. Further, mechanistic studies revealed that overexpression of DACH1 inhibited the growth and migration of HCC cell line, which were dependent in part on the inactivation of Wnt pathway via phosphorylation of GSK3β to suppress β-catenin. In agreement, the abundance of DACH1 was inversely correlated with several Wnt target genes. Collectively, our study indicated β-catenin is a novel target of DACH1 in HCC.


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